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Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics.
Inoue, Masatoshi; Takeuchi, Atsuya; Manita, Satoshi; Horigane, Shin-Ichiro; Sakamoto, Masayuki; Kawakami, Ryosuke; Yamaguchi, Kazushi; Otomo, Kouhei; Yokoyama, Hiroyuki; Kim, Ryang; Yokoyama, Tatsushi; Takemoto-Kimura, Sayaka; Abe, Manabu; Okamura, Michiko; Kondo, Yayoi; Quirin, Sean; Ramakrishnan, Charu; Imamura, Takeshi; Sakimura, Kenji; Nemoto, Tomomi; Kano, Masanobu; Fujii, Hajime; Deisseroth, Karl; Kitamura, Kazuo; Bito, Haruhiko.
Afiliação
  • Inoue M; Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Bioengineering, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Takeuchi A; Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Manita S; Department of Neurophysiology, Faculty of Medicine, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan.
  • Horigane SI; Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Neuroscience I, Research Institute of Environmental Medicine, Nagoya University, Nagoya, Aichi 464-8601, Japan; Department of Molecular/Cellular Neuroscience, Nagoya Un
  • Sakamoto M; Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Kawakami R; Laboratory of Molecular and Cellular Biophysics, Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan; Department of Molecular Medicine for Pathogenesis, Ehime University Graduate School of Medicine, Toon, Ehime 791-0295, Japan.
  • Yamaguchi K; Laboratory of Molecular and Cellular Biophysics, Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan.
  • Otomo K; Laboratory of Molecular and Cellular Biophysics, Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan.
  • Yokoyama H; New Industry Creation Hatchery Center (NICHe), Tohoku University, Sendai, Miyagi 980-8579, Japan.
  • Kim R; Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Yokoyama T; Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Takemoto-Kimura S; Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Neuroscience I, Research Institute of Environmental Medicine, Nagoya University, Nagoya, Aichi 464-8601, Japan; Department of Molecular/Cellular Neuroscience, Nagoya Un
  • Abe M; Department of Cellular Neurobiology, Brain Research Institute, Niigata University, Niigata, Niigata 951-8585, Japan.
  • Okamura M; Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Kondo Y; Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Quirin S; Department of Bioengineering, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Ramakrishnan C; Department of Bioengineering, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Imamura T; Department of Molecular Medicine for Pathogenesis, Ehime University Graduate School of Medicine, Toon, Ehime 791-0295, Japan.
  • Sakimura K; Department of Cellular Neurobiology, Brain Research Institute, Niigata University, Niigata, Niigata 951-8585, Japan.
  • Nemoto T; Laboratory of Molecular and Cellular Biophysics, Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan; Core Research for Evolutional Science and Technology (CREST), Japan Agency for Medical Research and Development, Tokyo, Japan.
  • Kano M; Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; International Research Center for Neurointelligence, The University of Tokyo, Tokyo, Japan.
  • Fujii H; Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Deisseroth K; Department of Bioengineering, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Kitamura K; Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Neurophysiology, Faculty of Medicine, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan.
  • Bito H; Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; International Research Center for Neurointelligence, The University of Tokyo, Tokyo, Japan. Electronic address: hbito@m.u-tokyo.ac.jp.
Cell ; 177(5): 1346-1360.e24, 2019 05 16.
Article em En | MEDLINE | ID: mdl-31080068
ABSTRACT
To decipher dynamic brain information processing, current genetically encoded calcium indicators (GECIs) are limited in single action potential (AP) detection speed, combinatorial spectral compatibility, and two-photon imaging depth. To address this, here, we rationally engineered a next-generation quadricolor GECI suite, XCaMPs. Single AP detection was achieved within 3-10 ms of spike onset, enabling measurements of fast-spike trains in parvalbumin (PV)-positive interneurons in the barrel cortex in vivo and recording three distinct (two inhibitory and one excitatory) ensembles during pre-motion activity in freely moving mice. In vivo paired recording of pre- and postsynaptic firing revealed spatiotemporal constraints of dendritic inhibition in layer 1 in vivo, between axons of somatostatin (SST)-positive interneurons and apical tufts dendrites of excitatory pyramidal neurons. Finally, non-invasive, subcortical imaging using red XCaMP-R uncovered somatosensation-evoked persistent activity in hippocampal CA1 neurons. Thus, the XCaMPs offer a critical enhancement of solution space in studies of complex neuronal circuit dynamics. VIDEO ABSTRACT.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Axônios / Potenciais de Ação / Córtex Cerebral / Células Piramidais / Hipocampo / Interneurônios Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Axônios / Potenciais de Ação / Córtex Cerebral / Células Piramidais / Hipocampo / Interneurônios Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article