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PB2 and hemagglutinin mutations confer a virulent phenotype on an H1N2 avian influenza virus in mice.
Yu, Zhijun; Ren, Zhiguang; Zhao, Yongkun; Cheng, Kaihui; Sun, Weiyang; Zhang, Xinghai; Wu, Jiaqiang; He, Hongbin; Xia, Xianzhu; Gao, Yuwei.
Afiliação
  • Yu Z; Institute of Poultry Science, Shandong Academy of Agricultural Sciences, No. 1 Jiaoxiao road, Jinan, 250023, Shandong, China. zhijun0215@gmail.com.
  • Ren Z; Joint National Laboratory for Antibody Drug Engineering, School of Basic Medical Sciences, Henan University, Kaifeng, 475004, China.
  • Zhao Y; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research Institute, Academy of Military Medical Science of PLA, 666 Liuyingxi St., Changchun, 130122, People's Republic of China.
  • Cheng K; Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, 250132, China.
  • Sun W; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research Institute, Academy of Military Medical Science of PLA, 666 Liuyingxi St., Changchun, 130122, People's Republic of China.
  • Zhang X; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research Institute, Academy of Military Medical Science of PLA, 666 Liuyingxi St., Changchun, 130122, People's Republic of China.
  • Wu J; Institute of Poultry Science, Shandong Academy of Agricultural Sciences, No. 1 Jiaoxiao road, Jinan, 250023, Shandong, China.
  • He H; College of Life Sciences, Shandong Normal University, Jinan, 250014, China.
  • Xia X; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research Institute, Academy of Military Medical Science of PLA, 666 Liuyingxi St., Changchun, 130122, People's Republic of China. xiaxzh@cae.cn.
  • Gao Y; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research Institute, Academy of Military Medical Science of PLA, 666 Liuyingxi St., Changchun, 130122, People's Republic of China. gaoyuwei@gmail.com.
Arch Virol ; 164(8): 2023-2029, 2019 Aug.
Article em En | MEDLINE | ID: mdl-31111259
We previously obtained mouse-adapted variants of H1N2 avian influenza virus that contained PB2-L134H, PB2-I647L, PB2-D701N, HA-G228S, and M1-D231N mutations. Here, we analyzed the effects of these mutations on viral pathogenicity in a mammalian model. By evaluating the virulence of mouse-adapted H1N2 variants at different generations, we found that the PB2-D701N and HA-G228S mutations both contribute to the virulence of this virus in mammals. Furthermore, we found that the PB2-D701N and HA-G228S mutations both enhance the ability of the virus to replicate in vivo and in vitro and that the PB2-D701N substitution results in an expansion of viral tissue tropism. These results suggest that the PB2-D701N mutation and the HA-G228S mutation are the major mammalian determinants of H1N2 virus. These results help us to understand more about the mechanisms by which influenza viruses adapt to mammals, and monitoring of these mutations can be used in continuous influenza surveillance to assess the pandemic potential of avian influenza virus variants.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Virais / Virulência / Glicoproteínas de Hemaglutininação de Vírus da Influenza / Vírus da Influenza A Subtipo H1N2 / Influenza Aviária / Mutação Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Virais / Virulência / Glicoproteínas de Hemaglutininação de Vírus da Influenza / Vírus da Influenza A Subtipo H1N2 / Influenza Aviária / Mutação Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article