Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals.
Methods Mol Biol
; 1996: 29-40, 2019.
Article
em En
| MEDLINE
| ID: mdl-31127544
Metabolic flux modeling is a complementary technique to standard metabolomics/metabonomics as routinely practiced in biological systems. Here we outline carbon-13-based isotopomer analysis using nuclear magnetic resonance as the primary analytical technique. Isotopomer analysis produces relative rates of tricarboxylic acid cycle turnover. If these measures are normalized to O2 consumption, absolute rates can be inferred. The primary biological system targeted in this review is cell culture.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Metabolômica
/
Análise do Fluxo Metabólico
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Espectroscopia de Prótons por Ressonância Magnética
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article