Your browser doesn't support javascript.
loading
Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals.
Ragavan, Mukundan; Merritt, Matthew E.
Afiliação
  • Ragavan M; Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, FL, USA.
  • Merritt ME; Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, FL, USA. matthewmerritt@ufl.edu.
Methods Mol Biol ; 1996: 29-40, 2019.
Article em En | MEDLINE | ID: mdl-31127544
Metabolic flux modeling is a complementary technique to standard metabolomics/metabonomics as routinely practiced in biological systems. Here we outline carbon-13-based isotopomer analysis using nuclear magnetic resonance as the primary analytical technique. Isotopomer analysis produces relative rates of tricarboxylic acid cycle turnover. If these measures are normalized to O2 consumption, absolute rates can be inferred. The primary biological system targeted in this review is cell culture.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Metabolômica / Análise do Fluxo Metabólico / Espectroscopia de Prótons por Ressonância Magnética Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Metabolômica / Análise do Fluxo Metabólico / Espectroscopia de Prótons por Ressonância Magnética Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article