Functional analysis of YY1 zinc fingers through cysteine mutagenesis.
FEBS Lett
; 593(12): 1392-1402, 2019 06.
Article
em En
| MEDLINE
| ID: mdl-31127623
As a transcription factor, Yin Yang 1 (YY1) either activates or represses gene expression depending on its recruited cofactors. The YY1 C-terminal consists of four zinc fingers (ZF) that are responsible for its DNA binding. However, the contribution of each YY1 ZF to its functions have not been fully elucidated. In this study, we used alanines to replace YY1 cysteines that are crucial to ZFs in binding to DNA. We characterized these YY1 mutants for their DNA binding, transcriptional activity, and functional role in maintaining MDA-MB-231 cell proliferation. We demonstrated that ZFs 2 and 3 are essential to the general biological activity of YY1. ZF 1 showed relatively low importance, while ZF 4 is virtually dispensable for YY1 function.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mutagênese
/
Dedos de Zinco
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Cisteína
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Fator de Transcrição YY1
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article