Separation of five diketopiperazines from the marine fungus Alternaria alternate HK-25 by high-speed counter-current chromatography.
J Sep Sci
; 42(15): 2510-2516, 2019 Aug.
Article
em En
| MEDLINE
| ID: mdl-31127863
ABSTRACT
High-speed counter-current chromatography was applied to the separation of five diketoperazines from the marine Alternaria alternate HK-25 for the first time using one-step elution method with a pair of two-phase solvent systems composed of petroleum ether/ethyl acetate/methanol/water (5.51157, v/v). Where 151.6 mg of crude sample yielded five diketoperazines, 12,13-dihydroxy-fumitremorgin C (1), gliotoxin (2), demethoxyfum itremorgin C (3), bisdethiobis(methylthio)gliotoxin (4), fumitremorgin C (5), and the purities of all compounds were above 94% as determined by high-performance liquid chromatography. The structures of these compounds were identified by 1 H and 13 C NMR spectroscopy. These results showed that high-speed counter-current chromatography can provide a feasible way for highly effective preparation of marine natural products, which ensured the supple of numerous samples for drug development.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Produtos Biológicos
/
Dicetopiperazinas
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Alternaria
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article