Nicking enzyme-assisted signal-amplifiable Hg2+ detection using upconversion nanoparticles.
Anal Chim Acta
; 1072: 75-80, 2019 Sep 23.
Article
em En
| MEDLINE
| ID: mdl-31146867
ABSTRACT
A highly specific and sensitive isothermal method for mercury detection using DNA-conjugated upconversion nanoparticles is reported. A single-stranded DNA containing thymine bases, used as the Hg2+-capturing element through the formation of thymine-Hg2+-thymine complex, is covalently attached to the NaYF4 Yb3+, Tm3+ nanoparticles. Luminescence resonance energy transfer takes place between the NaYF4 Yb3+, Tm3+ nanoparticles as donor and DNA-intercalating SYBR Green I as the acceptor upon excitation of 980â¯nm. The sensitivity and selectivity toward Hg2+ are enhanced using the nicking enzyme, Nt. Alwl, which leads to signal amplification. By monitoring the ratio of acceptor emission to a reference peak, the presence of Hg2+ ions are quantitatively determined with a lower detection limit of 0.14â¯nM, which is much lower than the US Environmental Protection Agency (EPA) limit of Hg2+ in drinking water.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Técnicas de Amplificação de Ácido Nucleico
/
Nanopartículas
/
Mercúrio
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article