Liquid mycobacterial culture outcomes after different sputum collection techniques before and during treatment.
Tuberculosis (Edinb)
; 116: 17-21, 2019 05.
Article
em En
| MEDLINE
| ID: mdl-31153513
ABSTRACT
SETTING:
Mycobacterial sputum culture is a key diagnostic and research tool.OBJECTIVE:
To compare mycobacterial culture outcomes of three sputum collection methods.DESIGN:
We compared culture results within sets of three sputum samples collected from 18 HIV-infected adult tuberculosis patients at regular intervals up to 84 days after treatment initiation. The first sputum was collected at home and brought to the clinic, where a second and third sputum were consecutively collected under supervision following mouthwash with bottled water and chlorhexidine solution respectively. All sputa were processed for liquid culture in duplicate.RESULTS:
Out of 556 cultures 430 (77.3%), 91 (16.4%) and 35 (6.3%) were positive, negative or contaminated, respectively. The odds of contamination were higher with home collection and with water rinse than with chlorhexidine rinse (OR 12.5, pâ¯<â¯0.001 and OR 6.7, pâ¯=â¯0.015). Chlorhexidine rinse increased the odds of a negative culture compared to water rinse (OR 3.5, pâ¯=â¯0.002). The odds of a positive culture were greater with water rinse than with home collection (OR 2.5, pâ¯=â¯0.005). Water rinse significantly reduced time to culture positivity.CONCLUSION:
Compared to sputum collected at home, chlorhexidine rinse reduces culture contamination and water rinse increases the rate and viable mycobacterial load of positive cultures.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Manejo de Espécimes
/
Escarro
/
Tuberculose Pulmonar
/
Técnicas Bacteriológicas
/
Mycobacterium tuberculosis
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Adult
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Female
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Humans
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Male
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article