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CKIP-1 regulates the immunomodulatory function of mesenchymal stem cells.
He, Youdi; Chen, Jun-Feng; Yang, Yan-Mei; Huang, Xiao-Hui; Dong, Xiao-Hui; Yang, Hui-Xin; Cao, Jun-Kai; Jiang, Xiao-Xia.
Afiliação
  • He Y; Department of Neurology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People's Republic of China.
  • Chen JF; Department of Stomatology, Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing, 100853, People's Republic of China.
  • Yang YM; Department of Stomatology, Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing, 100853, People's Republic of China.
  • Huang XH; Anhui Medical University, 81 Meishan Road, Hefei, 230032, People's Republic of China.
  • Dong XH; Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, 27 Taiping Road, Haidian District, Beijing, 100850, People's Republic of China.
  • Yang HX; Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, 27 Taiping Road, Haidian District, Beijing, 100850, People's Republic of China.
  • Cao JK; Department of Stomatology, Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing, 100853, People's Republic of China.
  • Jiang XX; Department of Stomatology, Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing, 100853, People's Republic of China. caojk@hotmail.com.
Mol Biol Rep ; 46(4): 3991-3999, 2019 Aug.
Article em En | MEDLINE | ID: mdl-31168669
ABSTRACT
Mesenchymal stem cells (MSCs) are self-renewing multipotent cells with immunoregulatory function, which makes them attractive candidates for regenerative medicine. However, the detailed mechanisms of their immunomodulatory capacity are not fully characterized. Here, we found that casein kinase 2 interacting protein-1 (CKIP-1) expression was induced in the murine MSC cell line C3H/10T1/2 by LPS. Knockdown of CKIP-1 did not cause significant differences on the cell cycle or immunophenotype of MSCs. However, MSCs with CKIP-1 knockdown showed enhanced immunosuppressive capacity. Real-time PCR and western blot analyses revealed that compared with the control group, MSCs with CKIP-1-knockdown exhibited higher IL-10 production and p38 MAPK phosphorylation following LPS treatment. Interestingly, the expression of CKIP-1 was decreased in MSCs following high glucose treatment. Furthermore, MSCs became more immunosuppressive after high glucose treatment, as shown by higher IL-10 production and enhanced inhibition of T cell proliferation. Collectively, our data reveal a novel role for CKIP-1 in regulating MSC-mediated immunomodulation, and indicate that MSCs become more immunosuppressive under high glucose conditions. These new insights may help in the development of future applications of MSCs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Transporte / Células-Tronco Mesenquimais / Fatores Imunológicos Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Transporte / Células-Tronco Mesenquimais / Fatores Imunológicos Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article