Automated analysis of mitomycin C in body fluids by high-performance liquid chromatography with on-line sample pre-treatment.
J Chromatogr
; 420(1): 53-62, 1987 Sep 04.
Article
em En
| MEDLINE
| ID: mdl-3117825
ABSTRACT
A fully automated liquid chromatographic system for the bioanalysis of mitomycin C has been described. The isolation of the analyte from the biological matrix (plasma, ascites and urine) is performed using a continuous-flow system equipped with a dialysis membrane in order to remove proteins. The samples are concentrated on a reversed-phase pre-column and subsequently introduced on to a reversed-phase analytical column by applying column-switching techniques. The drug is detected by absorbance measurements at 360 nm. Using the described system up to 100 samples a day can be analysed with determination limits of the order of 1 ng/ml, with a linear dynamic range of at least three decades for plasma and urine samples. The procedure was applied to pharmacokinetic studies of ovarian cancer patients treated intraperitoneally with mitomycin C.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Mitomicinas
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article