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Assessment of human pancreas cancer tissue and precursor lesions via a fluorophore with inherent PDAC selectivity.
Munhenzva, Ian R; Barth, Connor W; Sibrian-Vazquez, Martha; Wang, Lei G; Escobedo, Jorge O; Gibbs, Summer L; Strongin, Robert M.
Afiliação
  • Munhenzva IR; Department of Chemistry, Portland State University, 1719 SW 10th Avenue, Portland, OR 97201, United States.
  • Barth CW; Biomedical Engineering Department, Oregon Health & Science University, 3303 SW Bond Avenue, Portland, OR 97239, United States.
  • Sibrian-Vazquez M; Department of Chemistry, Portland State University, 1719 SW 10th Avenue, Portland, OR 97201, United States.
  • Wang LG; Biomedical Engineering Department, Oregon Health & Science University, 3303 SW Bond Avenue, Portland, OR 97239, United States.
  • Escobedo JO; Department of Chemistry, Portland State University, 1719 SW 10th Avenue, Portland, OR 97201, United States.
  • Gibbs SL; Biomedical Engineering Department, Oregon Health & Science University, 3303 SW Bond Avenue, Portland, OR 97239, United States; Knight Cancer Institute, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, United States; OHSU Center for Spatial Systems Biomed
  • Strongin RM; Department of Chemistry, Portland State University, 1719 SW 10th Avenue, Portland, OR 97201, United States; OHSU Center for Spatial Systems Biomedicine, Oregon Health & Science University, 2730 SW Moody Avenue, Portland, OR 97201, United States. Electronic address: strongin@pdx.edu.
Methods ; 168: 35-39, 2019 09 15.
Article em En | MEDLINE | ID: mdl-31185273
The current five-year survival rate of <5% for pancreatic ductal adenocarcinoma (PDAC) is compounded by late diagnosis, a lack of PDAC-specific intraoperative guidance to ensure complete resection, and the ineffectiveness of current therapies. Previously, utilizing compound 1, a fluorophore with inherent PDAC selectivity, PDAC was visualized both in vivo and ex vivo in a murine model. In the current study, human PDAC tissue is targeted. Compound 1 selectively stains ducts of the adenocarcinoma versus the surrounding stroma, enabling the imaging of PDAC in frozen tissue sections with high contrast. To enhance the potential of 1 for intraoperative applications, the ex vivo staining protocol was optimized for rapid margin assessment, with a final staining time of ~15 min. To measure diagnostic performance, the area under a receiver operating characteristic (ROC) curve was measured for the identification of ductal adenocarcinoma vs. stroma. The bright fluorescence contrast enabled quantitative determination of PDAC (or precancerous PanIN lesions) versus healthy pancreas tissue in human tissue array samples.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Pancreáticas / Carcinoma Ductal Pancreático / Imagem Óptica Tipo de estudo: Guideline / Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Pancreáticas / Carcinoma Ductal Pancreático / Imagem Óptica Tipo de estudo: Guideline / Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article