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The activation of adenylate cyclase by guanyl nucleotides in Saccharomyces cerevisiae is controlled by the CDC25 start gene product.
Daniel, J; Becker, J M; Enari, E; Levitzki, A.
Afiliação
  • Daniel J; Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
Mol Cell Biol ; 7(10): 3857-61, 1987 Oct.
Article em En | MEDLINE | ID: mdl-3119992
ABSTRACT
In the thermosensitive cdc25 start mutant of Saccharomyces cerevisiae, the regulation of adenylate cyclase by guanyl nucleotides was rapidly nullified when the enzyme was prepared from nonsynchronized cells shifted to the restrictive temperature. In agreement with previous in vivo complementation studies, this biochemical defect was fully suppressed by the expression of either the whole cloned CDC25 gene or its C-terminal portion. Moreover, membranes prepared from cdc25(Ts) cells grown at the permissive temperature evinced an altered regulation of adenylate cyclase by guanyl nucleotides. These results indicate that the CDC25 protein, together with RAS, is involved in the regulation of adenylate cyclase by guanyl nucleotides and raise the possibility that adenylate cyclase might form a ternary complex with RAS and CDC25.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Proteínas Fúngicas / Ciclo Celular / Adenilil Ciclases / Genes Fúngicos Idioma: En Ano de publicação: 1987 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Proteínas Fúngicas / Ciclo Celular / Adenilil Ciclases / Genes Fúngicos Idioma: En Ano de publicação: 1987 Tipo de documento: Article