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NF-YC12 is a key multi-functional regulator of accumulation of seed storage substances in rice.
Xiong, Yufei; Ren, Ye; Li, Wang; Wu, Fengsheng; Yang, Wenjie; Huang, Xiaolong; Yao, Jialing.
Afiliação
  • Xiong Y; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
  • Ren Y; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
  • Li W; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
  • Wu F; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
  • Yang W; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
  • Huang X; The Key Laboratory of Plant Physiology and Development Regulation, School of Life Sciences, Guizhou Normal University, Guiyang, China.
  • Yao J; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
J Exp Bot ; 70(15): 3765-3780, 2019 08 07.
Article em En | MEDLINE | ID: mdl-31211389
Starch and storage proteins, the primary storage substances of cereal endosperm, are a major source of food for humans. However, the transcriptional regulatory networks of the synthesis and accumulation of storage substances remain largely unknown. Here, we identified a rice endosperm-specific gene, NF-YC12, that encodes a putative nuclear factor-Y transcription factor subunit C. NF-YC12 is expressed in the aleurone layer and starchy endosperm during grain development. Knockout of NF-YC12 significantly decreased grain weight as well as altering starch and protein accumulation and starch granule formation. RNA-sequencing analysis revealed that in the nf-yc12 mutant genes related to starch biosynthesis and the metabolism of energy reserves were enriched in the down-regulated category. In addition, starch and protein contents in seeds differed between NF-YC12-overexpression lines and the wild-type. NF-YC12 was found to interact with NF-YB1. ChIP-qPCR and yeast one-hybrid assays showed that NF-YC12 regulated the rice sucrose transporter OsSUT1 in coordination with NF-YB1 in the aleurone layer. In addition, NF-YC12 was directly bound to the promoters of FLO6 (FLOURY ENDOSPERM6) and OsGS1;3 (glutamine synthetase1) in developing endosperm. This study demonstrates a transcriptional regulatory network involving NF-YC12, which coordinates multiple pathways to regulate endosperm development and the accumulation of storage substances in rice seeds.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Oryza / Sementes / Fatores de Transcrição Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Oryza / Sementes / Fatores de Transcrição Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2019 Tipo de documento: Article