Endovascular hypothermia improves post-resuscitation myocardial dysfunction by increasing mitochondrial biogenesis in a pig model of cardiac arrest.
Cryobiology
; 89: 6-13, 2019 08.
Article
em En
| MEDLINE
| ID: mdl-31283936
ABSTRACT
The aim of the study was to investigate the effects of endovascular hypothermia on mitochondrial biogenesis in a pig model of prolonged cardiac arrest (CA). Ventricular fibrillation was electrically induced, and animals were left untreated for 10â¯min; then after 6min of cardiopulmonary resuscitation (CPR), defibrillation was attempted. 25 animals that were successfully resuscitated were randomized into three groups Sham group (SG, 5, no CA), normal temperature group (NTG, 5 for 12â¯h observation and 5 for 24â¯h observation), and endovascular hypothermia group (EHG, 5 for 12â¯h observation and 5 for 24â¯h observation). The core temperatures (Tc) in the EHG were maintained at 34⯱â¯0.5⯰C for 6â¯h by an endovascular hypothermia device (Coolgard 3000), then actively increased at the speed of 0.5⯰C per hour during the next 6â¯h to achieve a normal body temperature, while Tc were maintained at 37.5⯱â¯0.5⯰C in the NTG. Cardiac and mitochondrial functions, the quantification of myocardial mitochondrial DNA (mtDNA), peroxisome proliferator-activated receptor coactivator-1α (PGC-1α), nuclear respiratory factor (NRF)-1, and NRF-2 were examined. Results showed that myocardial and mitochondrial injury and dysfunction increased significantly at 12â¯h and 24â¯h after CA. Endovascular hypothermia offered a method to rapidly achieve the target temperature and provide stable target temperature management (TTM). Cardiac outcomes were improved and myocardial injuries were alleviated with endovascular hypothermia. Compared with NTG, endovascular hypothermia significantly increased mitochondrial activity and biogenesis by amplifying mitochondrial biogenesis factors' expressions, including PGC-1α, NRF-1, and NRF-2. In conclusions, endovascular hypothermia after CA alleviated myocardial and mitochondrial dysfunction, and was associated with increasing mitochondrial biogenesis.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reanimação Cardiopulmonar
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Parada Cardíaca
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Hipotermia Induzida
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Mitocôndrias
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Miocárdio
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article