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Ancient Use of Ig Variable Domains Contributes Significantly to the TCRδ Repertoire.
Deiss, Thaddeus C; Breaux, Breanna; Ott, Jeannine A; Daniel, Rebecca A; Chen, Patricia L; Castro, Caitlin D; Ohta, Yuko; Flajnik, Martin F; Criscitiello, Michael F.
Afiliação
  • Deiss TC; Comparative Immunogenetics Laboratory, Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843.
  • Breaux B; Comparative Immunogenetics Laboratory, Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843.
  • Ott JA; Comparative Immunogenetics Laboratory, Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843.
  • Daniel RA; Comparative Immunogenetics Laboratory, Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843.
  • Chen PL; Comparative Immunogenetics Laboratory, Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843.
  • Castro CD; Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore, Baltimore, MD 21201; and.
  • Ohta Y; Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore, Baltimore, MD 21201; and.
  • Flajnik MF; Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore, Baltimore, MD 21201; and.
  • Criscitiello MF; Comparative Immunogenetics Laboratory, Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843; mcriscitiello@cvm.tamu.edu.
J Immunol ; 203(5): 1265-1275, 2019 09 01.
Article em En | MEDLINE | ID: mdl-31341077
ABSTRACT
The loci encoding B and T cell Ag receptors are generally distinct in commonly studied mammals, with each receptor's gene segments limited to intralocus, cis chromosomal rearrangements. The nurse shark (Ginglymostoma cirratum) represents the oldest vertebrate class, the cartilaginous fish, with adaptive immunity provided via Ig and TCR lineages, and is one species among a growing number of taxa employing Ig-TCRδ rearrangements that blend these distinct lineages. Analysis of the nurse shark Ig-TCRδ repertoire found that these rearrangements possess CDR3 characteristics highly similar to canonical TCRδ rearrangements. Furthermore, the Ig-TCRδ rearrangements are expressed with TCRγ, canonically found in the TCRδ heterodimer. We also quantified BCR and TCR transcripts in the thymus for BCR (IgHV-IgHC), chimeric (IgHV-TCRδC), and canonical (TCRδV-TCRδC) transcripts, finding equivalent expression levels in both thymus and spleen. We also characterized the nurse shark TCRαδ locus with a targeted bacterial artifical chromosome sequencing approach and found that the TCRδ locus houses a complex of V segments from multiple lineages. An IgH-like V segment, nestled within the nurse shark TCRδ translocus, grouped with IgHV-like rearrangements we found expressed with TCRδ (but not IgH) rearrangements in our phylogenetic analysis. This distinct lineage of TCRδ-associated IgH-like V segments was termed "TAILVs." Our data illustrate a dynamic TCRδ repertoire employing TCRδVs, NARTCRVs, bona fide trans-rearrangements from shark IgH clusters, and a novel lineage in the TCRδ-associated Ig-like V segments.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tubarões / Região Variável de Imunoglobulina / Receptores de Antígenos de Linfócitos T gama-delta / Domínios de Imunoglobulina Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tubarões / Região Variável de Imunoglobulina / Receptores de Antígenos de Linfócitos T gama-delta / Domínios de Imunoglobulina Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article