An arsenal of methods for the experimental characterization of intrinsically disordered proteins - How to choose and combine them?
Arch Biochem Biophys
; 676: 108055, 2019 11 15.
Article
em En
| MEDLINE
| ID: mdl-31356778
ABSTRACT
In this review, we detail the most common experimental approaches to assess and characterize protein intrinsic structural disorder, with the notable exception of NMR and EPR spectroscopy, two ideally suited approaches that will be described in depth in two other reviews within this special issue. We discuss the advantages, the limitations, as well as the caveats of the various methods. We also describe less common and more demanding approaches that enable achieving further insights into the conformational properties of IDPs. Finally, we present recent developments that have enabled assessment of structural disorder in living cells, and discuss the currently available methods to model IDPs as conformational ensembles.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Intrinsicamente Desordenadas
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article