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Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins.
Xue, Dongxiang; Xu, Tiandan; Wang, Huixia; Wu, Meng; Yuan, Ya; Wang, Wei; Tan, Qiwen; Zhao, Fei; Zhou, Fang; Hu, Tao; Jiang, Zhongxing; Liu, Zhi-Jie; Zhao, Suwen; Liu, Dongsheng; Wüthrich, Kurt; Tao, Houchao.
Afiliação
  • Xue D; iHuman Institute, ShanghaiTech University, Ren Building, 393 Middle Huaxia Road, Pudong New District, Shanghai, 201210, China.
  • Xu T; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Road, Shanghai, 201203, China.
  • Wang H; University of Chinese Academy of Sciences, No. 19A, Yuquan Road, Beijing, 100049, China.
  • Wu M; School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
  • Yuan Y; iHuman Institute, ShanghaiTech University, Ren Building, 393 Middle Huaxia Road, Pudong New District, Shanghai, 201210, China.
  • Wang W; University of Chinese Academy of Sciences, No. 19A, Yuquan Road, Beijing, 100049, China.
  • Tan Q; School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
  • Zhao F; CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China.
  • Zhou F; iHuman Institute, ShanghaiTech University, Ren Building, 393 Middle Huaxia Road, Pudong New District, Shanghai, 201210, China.
  • Hu T; University of Chinese Academy of Sciences, No. 19A, Yuquan Road, Beijing, 100049, China.
  • Jiang Z; School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
  • Liu ZJ; CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China.
  • Zhao S; iHuman Institute, ShanghaiTech University, Ren Building, 393 Middle Huaxia Road, Pudong New District, Shanghai, 201210, China.
  • Liu D; University of Chinese Academy of Sciences, No. 19A, Yuquan Road, Beijing, 100049, China.
  • Wüthrich K; School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
  • Tao H; CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China.
Chemistry ; 25(50): 11635-11640, 2019 Sep 06.
Article em En | MEDLINE | ID: mdl-31368214
ABSTRACT
Disulfide-containing detergents (DCDs) are introduced, which contain a disulfide bond in the hydrophobic tail. DCDs form smaller micelles than corresponding detergents with linear hydrocarbon chains, while providing good solubilization and reconstitution of membrane proteins. The use of this new class of detergents in structural biology is illustrated with solution NMR spectra of the human G protein-coupled receptor A2A AR, which is an α-helical protein, and the ß-barrel protein OmpX from E. coli.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas da Membrana Bacteriana Externa / Proteínas de Escherichia coli / Receptor A2A de Adenosina / Detergentes / Hidrolases Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas da Membrana Bacteriana Externa / Proteínas de Escherichia coli / Receptor A2A de Adenosina / Detergentes / Hidrolases Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article