[Analysis of infection status and recombination types of norovirus in patients with acute gastroenteritis in the Ningxia Hui Autonomous Region from 2016 to 2017].
Zhonghua Yu Fang Yi Xue Za Zhi
; 53(8): 811-816, 2019 Aug 06.
Article
em Zh
| MEDLINE
| ID: mdl-31378041
ABSTRACT
Objective:
To analyze the infection status and recombination of Norovirus in patients with acute gastroenteritis in Ningxia.Methods:
The specimens of 10 sentinel hospitals in Ningxia were collected from 2016 to 2017. Real-time quantitative PCR was used for nucleic acid detection. Gâ ¡-positive samples were amplified by RT-PCR for the RdRp and Capsid regions, then sequenced and genotyped. Evolution analysis was performed using software such as MEGA-X, and recombination analysis was performed using Simplot 3.5.1 and RDP4.Results:
The age of the 2 334 cases was 1.42 (0.68, 7.69) years old, 1 133 cases in 2016 and 1 201 cases in 2017, 1 343 and 991 cases for males and females respectively. The positive rate of Norovirus Gâ genogroup was 0.86% (20/2 334), and Gâ ¡ genogroup was 14.82% (346/2 334). A total of 78 recombinant strains were sequenced and 12 recombinant types were found. Gâ ¡.Pe/Gâ ¡.4Sydney_2012 and Gâ ¡.P12/Gâ ¡.3 were the main epidemic strains, accounting for 35.90% (28 strains) and 32.05% (25 strain) respectively, followed by Gâ ¡.P16/Gâ ¡.2 accounting for 12.82% (10 strains). Among them,Gâ ¡.P7/Gâ ¡.6 (2 strains), Gâ ¡.P12/Gâ ¡.3 (6 strains), Gâ ¡.P16/Gâ ¡.1 (2 strains), Gâ ¡.P16/Gâ ¡.2 (5 strains), Gâ ¡.Pe/Gâ ¡.4 (7 strains) were detected for the first time in Ningxia. Recombinant strains were all intergenotype recombination, and the recombination breakpionts were all located within ORF1.Conclusion:
Norovirus infection in Ningxia area was mainly in Gâ ¡ genogroup from 2016 to 2017, and most of them were recombinant strains. Gâ ¡.Pe/Gâ ¡.4Sydney_2012 and Gâ ¡.P12/Gâ ¡.3 were the main epidemic strains, followed by Gâ ¡.P16/Gâ ¡. 2.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
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Infecções por Caliciviridae
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Norovirus
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Gastroenterite
Limite:
Child
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Female
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Humans
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Male
País/Região como assunto:
Asia
Idioma:
Zh
Ano de publicação:
2019
Tipo de documento:
Article