High-level heterologous expression of the human transmembrane sterol Δ8,Δ7-isomerase in Pichia pastoris.
Protein Expr Purif
; 164: 105463, 2019 12.
Article
em En
| MEDLINE
| ID: mdl-31381990
ABSTRACT
Recombinant expression of human membrane proteins in large quantities remains a major challenge. Expression host is an important variable to screen for high-level production of membrane proteins. Using the green fluorescent protein (GFP) as a reporter, we screened the expression of a human multi-pass membrane protein called sterol Δ8-Δ7 isomerase in three different hosts Escherichia coli, Saccharomyces cerevisiae, and Pichia pastoris. The expression of the His-tagged isomerase was exceptionally high in P. pastoris, reaching ~200â¯mgâ¯L-1 in standard flasks, and ~1,000â¯mgâ¯L-1 in condensed culture that mimics fermentation. The heterogeneously expressed isomerase could be extracted fully with dodecyl maltoside, and the solubilized protein in the form of GFP fusion showed a sharp and symmetric peak on fluorescence-detection size exclusion chromatography. Our work provides a useful source for the purification of the recombinant isomerase.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pichia
/
Esteroide Isomerases
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article