Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice.
PLoS Biol
; 17(8): e3000374, 2019 08.
Article
em En
| MEDLINE
| ID: mdl-31393866
ABSTRACT
A deep understanding of how regulation of the multiple levels of gene expression in mammalian tissues give rise to complex phenotypes has been impeded by cellular diversity. A handful of techniques were developed to tag-select nucleic acids of interest in specific cell types, thereby enabling their capture. We expanded this strategy by developing the Tagger knock-in mouse line bearing a quad-cistronic transgene combining enrichment tools for nuclei, nascent RNA, translating mRNA, and mature microRNA (miRNA). We demonstrate that Tagger can capture the desired nucleic acids, enabling multiple omics approaches to be applied to specific cell types in vivo using a single transgenic mouse line.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácidos Nucleicos
/
Perfilação da Expressão Gênica
/
Sequenciamento Completo do Genoma
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article