Your browser doesn't support javascript.
loading
Determination of methyl order parameters using solid state NMR under off magic angle spinning.
Xue, Kai; Mamone, Salvatore; Koch, Benita; Sarkar, Riddhiman; Reif, Bernd.
Afiliação
  • Xue K; Helmholtz-Zentrum München (HMGU), Deutsches Forschungszentrum für Gesundheit und Umwelt, Ingolstädter Landstr. 1, 85764, Neuherberg, Germany.
  • Mamone S; Munich Center for Integrated Protein Science (CIPS-M) at Department Chemie, Technische Universität München (TUM), Lichtenbergstr. 4, 85747, Garching, Germany.
  • Koch B; Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
  • Sarkar R; Helmholtz-Zentrum München (HMGU), Deutsches Forschungszentrum für Gesundheit und Umwelt, Ingolstädter Landstr. 1, 85764, Neuherberg, Germany.
  • Reif B; Munich Center for Integrated Protein Science (CIPS-M) at Department Chemie, Technische Universität München (TUM), Lichtenbergstr. 4, 85747, Garching, Germany.
J Biomol NMR ; 73(8-9): 471-475, 2019 Sep.
Article em En | MEDLINE | ID: mdl-31407204
Quantification of dipolar couplings in biological solids is important for the understanding of dynamic processes. Under Magic Angle Spinning (MAS), order parameters are normally obtained by recoupling of anisotropic interactions involving the application of radio frequency pulses. We have recently shown that amide backbone order parameters can be estimated accurately in a spin-echo experiment in case the rotor spinning angle is slightly mis-calibrated. In this work, we apply this method to determine methyl order parameters in a deuterated sample of the SH3 domain of chicken α-spectrin in which the methyl containing side chains valine and leucine are selectively protonated.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anisotropia / Ressonância Magnética Nuclear Biomolecular Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anisotropia / Ressonância Magnética Nuclear Biomolecular Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article