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Ruthenium Complex Induce Cell Death in G-415 Gallbladder Cancer Cells.
Villota, Hernán; Pizarro, Sebastian; Gajardo, Francisco; Delgadillo, Álvaro; Cortés-Mancera, Fabián; Bernal, Giuliano.
Afiliação
  • Villota H; Laboratorio de Biología Molecular y Celular del Cáncer, CáncerLab, Departamento de Ciencias Biomédicas, Facultad de Medicina, Universidad Católica Del Norte, Larrondo 1281, Coquimbo, 1781421, Chile. hernan.villota@ucn.cl.
  • Pizarro S; Facultad de Ciencias Exactas y Aplicadas- GI2B, Instituto Tecnológico Metropolitano, 73 # 76A - 354 vía al volador, Medellín, Colombia. hernan.villota@ucn.cl.
  • Gajardo F; Departamento de Química, Facultad de Ciencias, Universidad de La Serena, Benavente 980, La Serena, Chile.
  • Delgadillo Á; Departamento de Química, Facultad de Ciencias, Universidad de La Serena, Benavente 980, La Serena, Chile.
  • Cortés-Mancera F; Departamento de Química, Facultad de Ciencias, Universidad de La Serena, Benavente 980, La Serena, Chile.
  • Bernal G; Facultad de Ciencias Exactas y Aplicadas- GI2B, Instituto Tecnológico Metropolitano, 73 # 76A - 354 vía al volador, Medellín, Colombia.
J Gastrointest Cancer ; 51(2): 571-578, 2020 Jun.
Article em En | MEDLINE | ID: mdl-31407248
ABSTRACT

PURPOSE:

In this work, we present a recently developed ruthenium complex that shows anticancer activity in gallbladder cancer cells.

METHODS:

After the synthesis of the new ruthenium complexes, the antiproliferative, cytotoxicity, and apoptosis activities were evaluated in vitro by the triple assay ApoTox-Glo. Then, the transcription levels of genes related to apoptosis were evaluated by real-time PCR (q-PCR).

RESULTS:

The ruthenium complex, called Ru-UCN3, inhibits the proliferation of gallbladder cancer cells G-415 by means of apoptosis, which was demonstrated by the overexpression of the pro-apoptotic genes Puma, Diablo, and Caspasa-9 together with the repression of the anti-apoptotic genes Bcl-xL and Bcl-2. In addition, we found strong caspase 3/7 activity in the cells at 24 h of the Ru-UCN3 exposure, which was evaluated by the triple ApoTox-Glo assay.

CONCLUSION:

The new ruthenium complexes evaluated had an inhibitory effect on G-415 cells. We think that Ru-UCN3 could be a promising anticancer agent, which should be explored with more in vitro and in vivo assays and probably with the chemical modulation of this molecule.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rutênio / Morte Celular / Neoplasias da Vesícula Biliar Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rutênio / Morte Celular / Neoplasias da Vesícula Biliar Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article