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Induced pluripotent stem cell line heterozygous for p.R501X mutation in filaggrin: KCLi003-A.
Kolundzic, Nikola; Khurana, Preeti; Devito, Liani; Jeriha, Jakob; Marx, Jennifer; Hobbs, Carl; Wong, Xuan Fei Colin Cornelius; Common, John E A; Liovic, Mirjana; Dubrac, Sandrine; Gruber, Robert; Schmuth, Matthias; Mauro, Theodora M; Ilic, Dusko.
Afiliação
  • Kolundzic N; Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK; Assisted Conception Unit, Guy's Hospital, London, UK.
  • Khurana P; Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK; Assisted Conception Unit, Guy's Hospital, London, UK.
  • Devito L; Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK; Assisted Conception Unit, Guy's Hospital, London, UK.
  • Jeriha J; Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK; Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
  • Marx J; Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK.
  • Hobbs C; Histology Laboratory, Wolfson Centre for Age-Related Diseases, School of Biomedical Sciences, King's College London, London, UK.
  • Wong XFCC; Skin Research Institute of Singapore, A*STAR, Singapore, Singapore.
  • Common JEA; Skin Research Institute of Singapore, A*STAR, Singapore, Singapore.
  • Liovic M; Medical Center for Molecular Biology, Faculty of Medicine, University of Ljubljana, Slovenia.
  • Dubrac S; Department of Dermatology, Venereology and Allergology, Medical University of Innsbruck, Innsbruck, Austria.
  • Gruber R; Department of Dermatology, Venereology and Allergology, Medical University of Innsbruck, Innsbruck, Austria.
  • Schmuth M; Department of Dermatology, Venereology and Allergology, Medical University of Innsbruck, Innsbruck, Austria.
  • Mauro TM; Dermatology Services, Veteran Affairs Medical Center, University of California San Francisco, San Francisco, CA, USA.
  • Ilic D; Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK; Assisted Conception Unit, Guy's Hospital, London, UK. Electronic address: dusko.ilic@kcl.ac.uk.
Stem Cell Res ; 39: 101527, 2019 08.
Article em En | MEDLINE | ID: mdl-31408836
ABSTRACT
We have generated an induced pluripotent stem cell (iPSC) line KCLi003-A (iOP101) from epidermal keratinocytes of a female donor, heterozygous for the loss-of-function mutation p.R501X in the filaggrin gene (FLG), using non-integrating Sendai virus vectors. Derivation and expansion of iPSCs were performed under xeno-free culture conditions. Characterization and validation of KCLi003-A line included molecular karyotyping, mutation screening using restriction enzyme digestion, next generation sequencing (NGS), while pluripotency and differentiation potential were confirmed by expression of associated markers in vitro and by in vivo teratoma assay.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas / Proteínas de Filamentos Intermediários Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
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XML
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Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas / Proteínas de Filamentos Intermediários Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article