Ferritin nanovehicle for targeted delivery of cytochrome C to cancer cells.
Sci Rep
; 9(1): 11749, 2019 08 13.
Article
em En
| MEDLINE
| ID: mdl-31409839
ABSTRACT
In this work, we have exploited the unique properties of a chimeric archaeal-human ferritin to encapsulate, deliver and release cytochrome c and induce apoptosis in a myeloid leukemia cell line. The chimeric protein combines the versatility in 24-meric assembly and cargo incorporation capability of Archaeglobus fulgidus ferritin with specific binding of human H ferritin to CD71, the "heavy duty" carrier responsible for transferrin-iron uptake. Delivery of ferritin-encapsulated cytochrome C to the Acute Promyelocytic Leukemia (APL) NB4 cell line, highly resistant to transfection by conventional methods, was successfully achieved in vitro. The effective liberation of cytochrome C within the cytosolic environment, demonstrated by double fluorescent labelling, induced apoptosis in the cancer cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucemia Promielocítica Aguda
/
Citocromos c
/
Nanoestruturas
/
Ferritinas
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article