Studying the Biotransformation of Phosphorothioate-Containing Oligonucleotide Drugs by LC-MS.
Methods Mol Biol
; 2036: 307-315, 2019.
Article
em En
| MEDLINE
| ID: mdl-31410805
ABSTRACT
Across the pharmaceutical industry, there is increasing interest and need to investigate the biotransformation of oligonucleotide drugs. The method of choice is high-resolution mass spectrometry due to its unmet sensitivity and specificity.Here, we describe a method developed and applied in our laboratory studying the biotransformation of phosphorothioate-containing oligonucleotide drugs. This method is based on capillary flow liquid chromatography with column switching coupled to high-resolution mass spectrometry.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida
/
Espectrometria de Massas em Tandem
/
Oligonucleotídeos Fosforotioatos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article