Oblique-plane single-molecule localization microscopy for tissues and small intact animals.
Nat Methods
; 16(9): 853-857, 2019 09.
Article
em En
| MEDLINE
| ID: mdl-31427757
ABSTRACT
Single-molecule localization microscopy (SMLM), while well established for cultured cells, is not yet fully compatible with tissue-scale samples. We introduce single-molecule oblique-plane microscopy (obSTORM), which by directly imaging oblique sections of samples with oblique light-sheet illumination offers a deep and volumetric SMLM platform that is convenient for standard tissue samples and small intact animals. We demonstrate super-resolution imaging at depths of up to 66 µm for cells, Caenorhabditis elegans gonads, Drosophila melanogaster larval brain, mouse retina and brain sections, and whole stickleback fish.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Retina
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Encéfalo
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Caenorhabditis elegans
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Drosophila melanogaster
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Peixes
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Imagem Individual de Molécula
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Microscopia de Fluorescência
Limite:
Animals
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Female
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Humans
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Male
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article