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Oblique-plane single-molecule localization microscopy for tissues and small intact animals.
Kim, Jeongmin; Wojcik, Michal; Wang, Yuan; Moon, Seonah; Zin, Emilia A; Marnani, Nadia; Newman, Zachary L; Flannery, John G; Xu, Ke; Zhang, Xiang.
Afiliação
  • Kim J; NSF Nanoscale Science and Engineering Center, University of California, Berkeley, Berkeley, CA, USA.
  • Wojcik M; Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Wang Y; Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA.
  • Moon S; NSF Nanoscale Science and Engineering Center, University of California, Berkeley, Berkeley, CA, USA.
  • Zin EA; Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Marnani N; Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA.
  • Newman ZL; Vision Science, School of Optometry, University of California, Berkeley, Berkeley, CA, USA.
  • Flannery JG; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
  • Xu K; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
  • Zhang X; Vision Science, School of Optometry, University of California, Berkeley, Berkeley, CA, USA.
Nat Methods ; 16(9): 853-857, 2019 09.
Article em En | MEDLINE | ID: mdl-31427757
ABSTRACT
Single-molecule localization microscopy (SMLM), while well established for cultured cells, is not yet fully compatible with tissue-scale samples. We introduce single-molecule oblique-plane microscopy (obSTORM), which by directly imaging oblique sections of samples with oblique light-sheet illumination offers a deep and volumetric SMLM platform that is convenient for standard tissue samples and small intact animals. We demonstrate super-resolution imaging at depths of up to 66 µm for cells, Caenorhabditis elegans gonads, Drosophila melanogaster larval brain, mouse retina and brain sections, and whole stickleback fish.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Retina / Encéfalo / Caenorhabditis elegans / Drosophila melanogaster / Peixes / Imagem Individual de Molécula / Microscopia de Fluorescência Limite: Animals / Female / Humans / Male Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
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PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Retina / Encéfalo / Caenorhabditis elegans / Drosophila melanogaster / Peixes / Imagem Individual de Molécula / Microscopia de Fluorescência Limite: Animals / Female / Humans / Male Idioma: En Ano de publicação: 2019 Tipo de documento: Article