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Colonization by B. infantis EVC001 modulates enteric inflammation in exclusively breastfed infants.
Henrick, Bethany M; Chew, Stephanie; Casaburi, Giorgio; Brown, Heather K; Frese, Steven A; Zhou, You; Underwood, Mark A; Smilowitz, Jennifer T.
Afiliação
  • Henrick BM; Evolve Biosystems, Inc, Davis, CA, USA. bhenrick2@unl.edu.
  • Chew S; Department of Food Science and Technology, University of Nebraska, Lincoln, NE, USA. bhenrick2@unl.edu.
  • Casaburi G; Evolve Biosystems, Inc, Davis, CA, USA.
  • Brown HK; Evolve Biosystems, Inc, Davis, CA, USA.
  • Frese SA; Evolve Biosystems, Inc, Davis, CA, USA.
  • Zhou Y; Evolve Biosystems, Inc, Davis, CA, USA.
  • Underwood MA; Department of Food Science and Technology, University of Nebraska, Lincoln, NE, USA.
  • Smilowitz JT; Morrison Microscopy Core Research Facility, University of Nebraska, Lincoln, NE, USA.
Pediatr Res ; 86(6): 749-757, 2019 12.
Article em En | MEDLINE | ID: mdl-31443102
BACKGROUND: Infant gut dysbiosis, often associated with low abundance of bifidobacteria, is linked to impaired immune development and inflammation-a risk factor for increased incidence of several childhood diseases. We investigated the impact of B. infantis EVC001 colonization on enteric inflammation in a subset of exclusively breastfed term infants from a larger clinical study. METHODS: Stool samples (n = 120) were collected from infants randomly selected to receive either 1.8 × 1010 CFU B. infantis EVC001 daily for 21 days (EVC001) or breast milk alone (controls), starting at day 7 postnatal. The fecal microbiome was analyzed using 16S ribosomal RNA, proinflammatory cytokines using multiplexed immunoassay, and fecal calprotectin using ELISA at three time points: days 6 (Baseline), 40, and 60 postnatal. RESULTS: Fecal calprotectin concentration negatively correlated with Bifidobacterium abundance (P < 0.0001; ρ = -0.72), and proinflammatory cytokines correlated with Clostridiaceae and Enterobacteriaceae, yet negatively correlated with Bifidobacteriaceae abundance. Proinflammatory cytokines were significantly lower in EVC001-fed infants on days 40 and 60 postnatally compared to baseline and compared to control infants. CONCLUSION: Our findings indicate that gut dysbiosis (absence of B. infantis) is associated with increased intestinal inflammation. Early addition of EVC001 to diet represents a novel strategy to prevent enteric inflammation during a critical developmental phase.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aleitamento Materno / Enterite / Bifidobacterium longum subspecies infantis Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Female / Humans / Male / Newborn Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aleitamento Materno / Enterite / Bifidobacterium longum subspecies infantis Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Female / Humans / Male / Newborn Idioma: En Ano de publicação: 2019 Tipo de documento: Article