Low-Voltage Flow-Through Electroporation Membrane and Method.
Methods Mol Biol
; 2050: 43-55, 2020.
Article
em En
| MEDLINE
| ID: mdl-31468478
ABSTRACT
Electroporation uses high electric field gradients to create pores within the membrane of living cells in order to deliver a substance, for example a gene, into the cytoplasm. To achieve such gradients, current electroporation devices deliver voltage pulses in the kV range to the cell medium. We describe here a new device based on gold-microtube membranes that can accomplish electroporation with voltage pulses that are orders of magnitude smaller, 4 V. The percentages of electroporated bacteria were found to be more than an order of magnitude higher than obtained with a commercial electroporator.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Membrana Celular
/
Eletroporação
/
Escherichia coli
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article