Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery.
J Vis Exp
; (150)2019 08 16.
Article
em En
| MEDLINE
| ID: mdl-31475987
ABSTRACT
Myocardial infarction (MI) and acute coronary diseases are among the most prominent causes of death in population with western lifestyle. The murine models of MI with permanent ligation of left-anterior descending (LAD) coronary artery closely mimics MI in humans. Murine models benefit from the extensive genetic engineering available nowadays. Here we propose a reproducible murine surgical model of myocardial infarction by permanent LAD coronary ligation. Our technique comprises anesthesia with ketamine/xylazine that can be rapidly reversed by administration of an antagonist, intubation without tracheotomy for mechanical-assisted ventilation, ventilation with application of extrinsic positive end-expiratory pressure (PEEP) to avoid alveolar collapse, a thoracotomy method limiting to the minimum surgical lesions made to skeletal muscles, and lung inflation without thoracentesis. This method is sparsely invasive, reproducible and reduces post-surgery mortality and complications.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vasos Coronários
/
Modelos Animais de Doenças
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Procedimentos Cirúrgicos Cardíacos
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Infarto do Miocárdio
Limite:
Animals
/
Humans
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Male
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article