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Polypyrrole/polylactic acid nanofibrous scaffold cotransplanted with bone marrow stromal cells promotes the functional recovery of spinal cord injury in rats.
Shu, Bing; Liu, Xue-Bin; Zhou, Jun-Feng; Huang, Hua; Wang, Jing-Yun; Sun, Xiao-Dan; Qin, Chuan; An, Yi-Hua.
Afiliação
  • Raynald; Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS), Comparative Medicine Centre, Peking Union Medical College (PUMC), Beijing, China.
  • Shu B; Department of Functional Neurosurgery, The Third Medical Centre, Chinese PLA (People's Liberation Army) General Hospital, Beijing, China.
  • Liu XB; Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, Beijing, China.
  • Zhou JF; Department of Functional Neurosurgery, The Third Medical Centre, Chinese PLA (People's Liberation Army) General Hospital, Beijing, China.
  • Huang H; A State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, China.
  • Wang JY; Key Laboratory of Advanced Materials of Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University, Beijing, China.
  • Sun XD; Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.
  • Qin C; A State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, China.
  • An YH; Key Laboratory of Advanced Materials of Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University, Beijing, China.
CNS Neurosci Ther ; 25(9): 951-964, 2019 09.
Article em En | MEDLINE | ID: mdl-31486601
ABSTRACT

AIMS:

The objective of this study was to analyze the efficacy of polypyrrole/polylactic acid (PPy/PLA) nanofibrous scaffold cotransplanted with bone marrow stromal cells (BMSCs) in promoting the functional recovery in a rat spinal cord injury (SCI).

METHODS:

Female Sprague-Dawley rats were randomly divided into three groups (n = 18/group) control group, PPy/PLA group, and PPy/PLA/BMSCs group. The SCI was induced in all rats. Consequently, rats in PPy/PLA/BMSCs group were transplanted with 1 × 105 BMSCs after implantation of PPy/PLA, while those in the PPy/PLA group were implanted with PPy/PLA only; no implantation was performed in the control group. Six weeks after surgery, immunofluorescence microscopy, electron microscope, and polymerase chain reaction (PCR) techniques were performed to assess the changes in the injured spinal cord tissues.

RESULTS:

Electrophysiology and locomotor function testing suggested that PPy/PLA nanofibrous scaffold cotransplanted with BMSCs could promote the functional recovery of the spinal cord. Six weeks after the operation, lower amount of scar tissue was found in the PPy/PLA group compared with the control group. Abundant neurofilament (NF) and neuron-specific marker (NeuN) positive staining, and myelin formations were detected in the injured area. In addition, the transplantation of BMSCs not only improved the efficacy of PPy/PLA but also managed to survive well and was differentiated into neural and neuroglial cells.

CONCLUSIONS:

The implantation of PPy/PLA nanofibrous scaffold and BMSCs has a great potential to restore the electrical conduction and to promote functional recovery by inhibiting the scar tissue formation, promoting axon regeneration, and bridging the gap lesion.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismos da Medula Espinal / Recuperação de Função Fisiológica / Transplante de Células-Tronco Mesenquimais / Alicerces Teciduais / Nanofibras / Células-Tronco Mesenquimais Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismos da Medula Espinal / Recuperação de Função Fisiológica / Transplante de Células-Tronco Mesenquimais / Alicerces Teciduais / Nanofibras / Células-Tronco Mesenquimais Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article