Circular RNA ciRS-7 inhibits autophagy of ESCC cells by functioning as miR-1299 sponge to target EGFR signaling.
J Cell Biochem
; 121(2): 1039-1049, 2020 02.
Article
em En
| MEDLINE
| ID: mdl-31490018
ABSTRACT
Autophagy is a kind of intracellular degradation pathway which could be regulated by many noncoding RNAs. ciRS-7, also called CDR1as, is a circular RNA that is relatively well studied at present. In our recent study, we have found that the expression of ciRS-7 is abnormally increased in the esophageal squamous cell carcinoma (ESCC), and may function as an oncogene to accelerate ESCC progression through sponging miR-876-5p. Meanwhile, another study showed that ciRS-7 is highly expressed in the triple-negative breast cancer (TNBC) and may function as a competing endogenous RNA of miR-1299 to maintain the high migration and invasive capacity of TNBC cells. Of interest, in the present work, we observed that ciRS-7 could inhibit starvation or rapamycin-induced autophagy of ESCC cells and miR-1299 promotes starvation or rapamycin-induced autophagy of ESCC cells. Mechanically, miR-1299 could directly bind to the 3'-untranslated region of epidermal growth factor receptor (EGFR) and then affects its downstream Akt-mTOR pathway in ESCC cells. Consistent with our past findings, ciRS-7 could also sponge miR-1299 in ESCC cells. Taken together, this study has shed light on that circular RNA ciRS-7 inhibits autophagy of ESCC cells by functioning as miR-1299 sponge to target EGFR signaling.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Autofagia
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Biomarcadores Tumorais
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Regulação Neoplásica da Expressão Gênica
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MicroRNAs
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Carcinoma de Células Escamosas do Esôfago
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RNA Circular
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article