Pumpkin polysaccharide preparation, simulated gastrointestinal digestion, and in vivo biodistribution.
Int J Biol Macromol
; 141: 1293-1303, 2019 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-31499116
ABSTRACT
We employed a single factor and response surface methodology based on Box-Behnken design (BBD) to optimize the extraction of pumpkin polysaccharides. We then simulated pumpkin polysaccharide gastrointestinal digestion in vitro and investigated their biodistribution in mice. The optimal extraction conditions, with a yield of 7.38⯱â¯0.21%, were as follows a concentration of NaOH 1.20%, a ratio of material to liquid of 111, and an extraction time of 2.1â¯h, respectively, according to single factor and BBD experiments. In the gastrointestinal experiment in vitro, the molecular weight of the polysaccharides markedly decreased after gastric digestion for 30â¯min, suggesting the decline is due to the breakdown of polysaccharide glycosidic bonds. The simulated intestinal fluid had little effect on polysaccharides digestion within 240â¯min. Analysis of the biodistribution in mice indicated that the polysaccharides distribute in the duodenum, jejunum, and ileum 30 to 60â¯min after intragastrical administration, and are absorbed in the jejunum and ileum after 60 to 360â¯min. These results provide information on the digestion and biodistribution of pumpkin polysaccharides and offer a theoretical basis for further understanding the absorption mechanisms in vivo.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Cucurbita
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Biomimética
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Trato Gastrointestinal
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Digestão
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article