Iron oxide nanoparticle-calcium phosphate cement enhanced the osteogenic activities of stem cells through WNT/ß-catenin signaling.
Mater Sci Eng C Mater Biol Appl
; 104: 109955, 2019 Nov.
Article
em En
| MEDLINE
| ID: mdl-31500064
ABSTRACT
Calcium phosphate cement (CPC), functionalized with iron oxide nanoparticles (IONP), is of great promise to promote osteoinduction and new bone formation. In this work, the IONP powder was added into the CPC powder to fabricate CPCâ¯+â¯IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored. A series of CPCâ¯+â¯IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid. Western blotting and RT-PCR were used to analyze the signaling pathway. The IONP incorporation substantially enhanced the performance of CPCâ¯+â¯IONP, with increases in both mechanical strength and cellular activities. The IONP addition greatly promoted the osteogenesis of hDPSCs, elevating the ALP activity, the expression of osteogenic marker genes and bone matrix formation with 1.5-2-fold increases. The 3% IONP incorporation showed the most enhancement among all groups. Activation of the extracellular signal-related kinases WNT/ß-catenin in DPSCs was observed, and this activation was attenuated by the WNT inhibitor DKK1. The results indicated that the osteogenic behavior of hDPSCs was likely driven by CPCâ¯+â¯IONP via the WNT signaling pathway. In conclusion, incorporate IONP into CPC scaffold remarkably enhanced the spreading, osteogenic differentiation and bone mineral synthesis of stem cell. Therefore, this method had great potential for bone tissue engineering. The novel CPCâ¯+â¯IONP composite scaffolds with stem cells are promising to provide an innovative strategy to enhance bone regenerative therapies.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteogênese
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Células-Tronco
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Cimentos Ósseos
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Fosfatos de Cálcio
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Compostos Férricos
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Transdução de Sinais
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Nanopartículas
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article