Multiparameter flow cytometry analysis of leukocyte markers for diagnosis in preterm neonatal sepsis.
J Matern Fetal Neonatal Med
; 34(14): 2323-2333, 2021 Jul.
Article
em En
| MEDLINE
| ID: mdl-31537145
ABSTRACT
PURPOSE:
Neonatal sepsis is an important public health concern worldwide due to its immediate lethality and long-term morbidity rates, Clinical evaluation and laboratory analyses are indispensable for diagnosis of neonatal sepsis. However, assessing multiple biomarkers in neonates is difficult due to limited blood availability. The aim is to investigate if the neonatal sepsis in preterm could be identified by multiparameter analysis with flow cytometry. MATERIALS ANDMETHODS:
The expression of activation-related molecules was evaluated by flow cytometry in newborn with or without risk factors for sepsis.RESULTS:
Our analysis revealed that several markers could be useful for sepsis diagnosis, such as CD45RA, CD45RO, or CD71 on T cells; HLA-DR on NKT or classic monocytes, and TREM-1 on non-classic monocytes or neutrophils. However, ROC analysis shows that the expression of CD45RO on T lymphocytes is the only useful biomarker for diagnosis of neonatal late-onset sepsis. Also, decision tree analyses showed that CD45RO plus CD27 could help differentiate the preterm septic neonates from those with risk factors.CONCLUSIONS:
Our study shows a complementary and practical strategy for biomarker assessment in neonatal sepsis.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sepse
/
Sepse Neonatal
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Humans
/
Newborn
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article