Dual Site-Specific Labeling of an Antibody Fragment through Sortase A and π-Clamp Conjugation.
Bioconjug Chem
; 30(10): 2539-2543, 2019 10 16.
Article
em En
| MEDLINE
| ID: mdl-31560523
ABSTRACT
The functionalization of proteins with different cargo molecules is highly desirable for a broad range of applications. However, the reproducible production of defined conjugates with multiple functionalities is a significant challenge. Herein, we report the dual site-specific labeling of an antibody fragment, utilizing the orthogonal Sortase A and π-clamp conjugation methods, and demonstrate that binding of the antibody fragment to its target receptor is retained after dual labeling.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Fragmentos de Imunoglobulinas
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Cisteína Endopeptidases
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Aminoaciltransferases
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article