A mesophilic cysteine-less split intein for protein <i>trans</i>-splicing applications under oxidizing conditions.

Bhagawati, Maniraj; Terhorst, Tobias M E; Füsser, Friederike; Hoffmann, Simon; Pasch, Tim; Pietrokovski, Shmuel; Mootz, Henning D

A mesophilic cysteine-less split intein for protein trans-splicing applications under oxidizing conditions.

Bhagawati, Maniraj; Terhorst, Tobias M E; Füsser, Friederike; Hoffmann, Simon; Pasch, Tim; Pietrokovski, Shmuel; Mootz, Henning D.

Afiliação

Bhagawati M; Institute of Biochemistry, University of Münster, 48149 Münster, Germany.
Terhorst TME; Institute of Biochemistry, University of Münster, 48149 Münster, Germany.
Füsser F; Institute of Biochemistry, University of Münster, 48149 Münster, Germany.
Hoffmann S; Institute of Biochemistry, University of Münster, 48149 Münster, Germany.
Pasch T; Institute of Biochemistry, University of Münster, 48149 Münster, Germany.
Pietrokovski S; Department of Molecular Genetics, Weizmann Institute of Science, 76100 Rehovot, Israel shmuel.pietrokovski@weizmann.ac.il Henning.Mootz@uni-muenster.de.
Mootz HD; Institute of Biochemistry, University of Münster, 48149 Münster, Germany; shmuel.pietrokovski@weizmann.ac.il Henning.Mootz@uni-muenster.de.

Proc Natl Acad Sci U S A ; 116(44): 22164-22172, 2019 10 29.

Article em En | MEDLINE | ID: mdl-31611397

Assuntos

Inteínas/fisiologia; Processamento de Proteína; Sequência de Aminoácidos; Cisteína; Engenharia Genética; Células HeLa; Humanos; Oxirredução; Fragmentos de Peptídeos/química; Temperatura

Palavras-chave

chemical biology; fluorescent labeling; protein ligation; thiol bioconjugation

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Processamento de Proteína / Inteínas Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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