Monitoring glycation levels of a bispecific monoclonal antibody at subunit level by ultrahigh-resolution MALDI FT-ICR mass spectrometry.

Gstöttner, Christoph; Reusch, Dietmar; Haberger, Markus; Dragan, Irina; Van Veelen, Peter; Kilgour, David P A; Tsybin, Yury O; van der Burgt, Yuri E M; Wuhrer, Manfred; Nicolardi, Simone

Gstöttner, Christoph; Reusch, Dietmar; Haberger, Markus; Dragan, Irina; Van Veelen, Peter; Kilgour, David P A; Tsybin, Yury O; van der Burgt, Yuri E M; Wuhrer, Manfred; Nicolardi, Simone.

Afiliação

Gstöttner C; Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands.
Reusch D; Pharma Technical Development Penzberg, Roche Diagnostics GmbH, Penzberg, Germany.
Haberger M; Pharma Technical Development Penzberg, Roche Diagnostics GmbH, Penzberg, Germany.
Dragan I; Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands.
Van Veelen P; Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands.
Kilgour DPA; Department of Chemistry, Nottingham Trent University, Nottingham, U.K.
Tsybin YO; Spectroswiss, EPFL Innovation Park, Lausanne, Switzerland.
van der Burgt YEM; Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands.
Wuhrer M; Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands.
Nicolardi S; Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands.

MAbs ; 12(1): 1682403, 2020.

Article em En | MEDLINE | ID: mdl-31630606

Assuntos

Anticorpos Biespecíficos/química; Antineoplásicos Imunológicos/química; Bioengenharia/métodos; Subunidades de Imunoglobulinas/química; Angiotensinas/imunologia; Animais; Ciclotrons; Análise de Fourier; Glicosilação; Humanos; Camundongos; Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz; Fator A de Crescimento do Endotélio Vascular/imunologia

Palavras-chave

Bispecific monoclonal antibody; FT-ICR mass spectrometry; IdeS digestion; MALDI-ISD; PTM; glycation; middle-down; middle-up; post-translational modification

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anticorpos Biespecíficos / Subunidades de Imunoglobulinas / Bioengenharia / Antineoplásicos Imunológicos Limite: Animals / Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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