Your browser doesn't support javascript.
loading
Reversible and Selective Interconversion of Hydrogen and Carbon Dioxide into Formate by a Semiartificial Formate Hydrogenlyase Mimic.
Sokol, Katarzyna P; Robinson, William E; Oliveira, Ana R; Zacarias, Sonia; Lee, Chong-Yong; Madden, Christopher; Bassegoda, Arnau; Hirst, Judy; Pereira, Inês A C; Reisner, Erwin.
Afiliação
  • Sokol KP; Department of Chemistry , University of Cambridge , Lensfield Road , Cambridge CB2 1EW , U.K.
  • Robinson WE; Department of Chemistry , University of Cambridge , Lensfield Road , Cambridge CB2 1EW , U.K.
  • Oliveira AR; Instituto de Tecnologia Química e Biológica António Xavier (ITQB) , Universidade NOVA de Lisboa , Av. da República , 2780-157 Oeiras , Portugal.
  • Zacarias S; Instituto de Tecnologia Química e Biológica António Xavier (ITQB) , Universidade NOVA de Lisboa , Av. da República , 2780-157 Oeiras , Portugal.
  • Lee CY; Department of Chemistry , University of Cambridge , Lensfield Road , Cambridge CB2 1EW , U.K.
  • Madden C; Department of Chemistry , University of Cambridge , Lensfield Road , Cambridge CB2 1EW , U.K.
  • Bassegoda A; Medical Research Council Mitochondrial Biology Unit , University of Cambridge , The Keith Peters Building, Cambridge Biomedical Campus, Hills Road , Cambridge CB2 0XY , U.K.
  • Hirst J; Medical Research Council Mitochondrial Biology Unit , University of Cambridge , The Keith Peters Building, Cambridge Biomedical Campus, Hills Road , Cambridge CB2 0XY , U.K.
  • Pereira IAC; Instituto de Tecnologia Química e Biológica António Xavier (ITQB) , Universidade NOVA de Lisboa , Av. da República , 2780-157 Oeiras , Portugal.
  • Reisner E; Department of Chemistry , University of Cambridge , Lensfield Road , Cambridge CB2 1EW , U.K.
J Am Chem Soc ; 141(44): 17498-17502, 2019 11 06.
Article em En | MEDLINE | ID: mdl-31638793
The biological formate hydrogenlyase (FHL) complex links a formate dehydrogenase (FDH) to a hydrogenase (H2ase) and produces H2 and CO2 from formate via mixed-acid fermentation in Escherichia coli. Here, we describe an electrochemical and a colloidal semiartificial FHL system that consists of an FDH and a H2ase immobilized on conductive indium tin oxide (ITO) as an electron relay. These in vitro systems benefit from the efficient wiring of a highly active enzyme pair and allow for the reversible conversion of formate to H2 and CO2 under ambient temperature and pressure. The hybrid systems provide a template for the design of synthetic catalysts and surpass the FHL complex in vivo by storing and releasing H2 on demand by interconverting CO2/H2 and formate with minimal bias in either direction.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article