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Circular RNA LARP4 correlates with decreased Enneking stage, better histological response, and prolonged survival profiles, and it elevates chemosensitivity to cisplatin and doxorubicin via sponging microRNA-424 in osteosarcoma.
Hu, Yuhang; Gu, Jiaao; Shen, Hongtao; Shao, Tuo; Li, Song; Wang, Wei; Yu, Zhange.
Afiliação
  • Hu Y; Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Gu J; Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Shen H; Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Shao T; Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Li S; Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Wang W; Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Yu Z; Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
J Clin Lab Anal ; 34(2): e23045, 2020 Feb.
Article em En | MEDLINE | ID: mdl-31642110
BACKGROUND: This study aimed to evaluate the association of circular RNA La-related RNA-binding protein 4 (circ-LARP4) with clinical features and prognosis in osteosarcoma patients, and further explore its effect on chemosensitivity in osteosarcoma cells. METHODS: Seventy-two osteosarcoma patients with Enneking stage IIA-IIB who underwent resection were consecutively enrolled, and then, tumor tissues and non-tumor tissues were obtained. Circ-LARP4 in tumor tissue/non-tumor tissue was detected by quantitative polymerase chain reaction. After circ-LARP4 overexpression and negative control overexpression plasmid transfection, relative cell viability (%) was evaluated by Cell Counting Kit-8 in MG63 cells treated by different concentrations of cisplatin, methotrexate, and doxorubicin, and IC50 was calculated. RESULTS: Circ-LARP4 was downregulated in tumor tissue compared with non-tumor tissue and had a good value in distinguishing tumor tissue from non-tumor tissue with an area under curve of 0.829 (95% CI: 0.762-0.859). Meanwhile, tumor circ-LARP4 was negatively correlated with the Enneking stage. After resection, circ-LARP4 high expression patients showed an increased tumor cell necrosis rate to adjuvant chemotherapy compared to circ-LARP4 low expression patients, and circ-LARP4 high expression correlated with prolonged disease-free survival and overall survival. In vitro experiments revealed that circ-LARP4 overexpression elevated the chemosensitivity of MG63 cells to cisplatin and doxorubicin but not methotrexate, with decreased cisplatin IC50 and doxorubicin IC50 concentrations than negative control. Besides, miR-424 overexpression attenuated the chemosensitivity in circ-LARP4 overexpression-treated MG63 cells. CONCLUSION: Circ-LARP4 high expression correlates with decreased Enneking stage and prolonged survival profiles, and it elevates chemosensitivity to cisplatin and doxorubicin via sponging miR-424 in osteosarcoma.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ribonucleoproteínas / Autoantígenos / Neoplasias Ósseas / Osteossarcoma / Resistencia a Medicamentos Antineoplásicos / MicroRNAs / RNA Circular Limite: Adolescent / Adult / Child / Female / Humans / Male Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ribonucleoproteínas / Autoantígenos / Neoplasias Ósseas / Osteossarcoma / Resistencia a Medicamentos Antineoplásicos / MicroRNAs / RNA Circular Limite: Adolescent / Adult / Child / Female / Humans / Male Idioma: En Ano de publicação: 2020 Tipo de documento: Article