Gene cloning and characterization of thiourocanate hydratase from Burkholderia sp. HME13.

Muramatsu, Hisashi; Miyaoku, Haruna; Kurita, Syuya; Matsuo, Hidenori; Kashiwagi, Takehiro; Kim, Chul-Sa; Hayashi, Motoko; Yamamoto, Hiroaki; Kato, Shin-Ichiro; Nagata, Shinji

Muramatsu, Hisashi; Miyaoku, Haruna; Kurita, Syuya; Matsuo, Hidenori; Kashiwagi, Takehiro; Kim, Chul-Sa; Hayashi, Motoko; Yamamoto, Hiroaki; Kato, Shin-Ichiro; Nagata, Shinji.

Afiliação

Muramatsu H; Multidisciplinary Science Cluster, Research and Education Faculty, Kochi University, B200 Monobe, Nankoku, Kochi 783-8502, Japan.
Miyaoku H; Department of Agriculture, Kochi University, B200 Monobe, Nankoku, Kochi 783-8502, Japan.
Kurita S; Department of Agriculture, Kochi University, B200 Monobe, Nankoku, Kochi 783-8502, Japan.
Matsuo H; Graduate School of Integrated Arts and Sciences, Kochi University, B200 Monobe, Nankoku, Kochi 783-8502, Japan.
Kashiwagi T; Multidisciplinary Science Cluster, Research and Education Faculty, Kochi University, B200 Monobe, Nankoku, Kochi 783-8502, Japan.
Kim CS; Multidisciplinary Science Cluster, Research and Education Faculty, Kochi University, B200 Monobe, Nankoku, Kochi 783-8502, Japan.
Hayashi M; Corporate Research Center, R&D Headquaters Management, Daicel Corporation 1-1 Shinko-cho, Myoko, shi, Niigata 944-8550, Japan.
Yamamoto H; Corporate Research Center, R&D Headquaters Management, Daicel Corporation 1-1 Shinko-cho, Myoko, shi, Niigata 944-8550, Japan.
Kato SI; Multidisciplinary Science Cluster, Research and Education Faculty, Kochi University, B200 Monobe, Nankoku, Kochi 783-8502, Japan.
Nagata S; Multidisciplinary Science Cluster, Research and Education Faculty, Kochi University, B200 Monobe, Nankoku, Kochi 783-8502, Japan.

J Biochem ; 167(3): 333-341, 2020 Mar 01.

Article em En | MEDLINE | ID: mdl-31725161

Assuntos

Burkholderia/enzimologia; Hidroliases/metabolismo; Sequência de Aminoácidos; Burkholderia/genética; Catálise; Clonagem Molecular; Cobre/química; Escherichia coli/metabolismo; Hidroliases/antagonistas & inibidores; Hidroliases/química; Hidroliases/genética; Concentração de Íons de Hidrogênio; Cinética; Espectrometria de Massas; Cloreto de Mercúrio/química; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Especificidade por Substrato; Temperatura; Urocanato Hidratase/genética

Palavras-chave

Abbreviations: 2-ME; Burkholdria; 2-mercaptoethanol; IPTG; LB; Luria-Bertani; PMSF; UPLC; ergothioneine; isopropyl-ß-d-thiogalactopyranoside; phenylmethylsulfonyl fluoride; thiourocanate hydratase; thiourocanic acid; ultra-performance liquid chromatography; urocanase

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Burkholderia / Hidroliases Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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