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Laccases: structure, function, and potential application in water bioremediation.
Arregui, Leticia; Ayala, Marcela; Gómez-Gil, Ximena; Gutiérrez-Soto, Guadalupe; Hernández-Luna, Carlos Eduardo; Herrera de Los Santos, Mayra; Levin, Laura; Rojo-Domínguez, Arturo; Romero-Martínez, Daniel; Saparrat, Mario C N; Trujillo-Roldán, Mauricio A; Valdez-Cruz, Norma A.
Afiliação
  • Arregui L; Departamento de Ciencias Naturales, Universidad Autónoma Metropolitana, Unidad Cuajimalpa, Av. Vasco de Quiroga 4871, Col. Santa Fe Cuajimalpa, C.P. 05348, Mexico City, Mexico.
  • Ayala M; Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001 Chamilpa, 62210, Cuernavaca, Morelos, Mexico.
  • Gómez-Gil X; Programa de Investigación de Producción de Biomoléculas, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP. 70228, Mexico City, CP. 04510, Mexico.
  • Gutiérrez-Soto G; Facultad de Agronomía, Universidad Autónoma de Nuevo León, Francisco Villa, 66059 Colonia Ex hacienda El Canadá, General Escobedo, Nuevo León, Mexico.
  • Hernández-Luna CE; Laboratorio de Enzimología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Pedro de Alba y Manuel L. Barragán, Cd. Universitaria, 66451, San Nicolás de los Garza, Nuevo León, Mexico.
  • Herrera de Los Santos M; Programa de Investigación de Producción de Biomoléculas, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP. 70228, Mexico City, CP. 04510, Mexico.
  • Levin L; Laboratorio de Micología Experimental, DBBE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, INMIBO-CONICET, Ciudad Universitaria, Pabellón 2, Piso 4, C1428BGA, Ciudad Autónoma de Buenos Aires, Argentina.
  • Rojo-Domínguez A; Departamento de Ciencias Naturales, Universidad Autónoma Metropolitana, Unidad Cuajimalpa, Av. Vasco de Quiroga 4871, Col. Santa Fe Cuajimalpa, C.P. 05348, Mexico City, Mexico.
  • Romero-Martínez D; Programa de Investigación de Producción de Biomoléculas, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP. 70228, Mexico City, CP. 04510, Mexico.
  • Saparrat MCN; Instituto de Fisiología Vegetal (INFIVE), Universidad Nacional de La Plata (UNLP)-CCT-La Plata-Consejo Nacional de Investigaciones Científicas y técnicas (CONICET), Diag. 113 y 61, 327CC, 1900, La Plata, Argentina.
  • Trujillo-Roldán MA; Instituto de Botánica Spegazzini, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, 53 # 477, 1900, La Plata, Argentina.
  • Valdez-Cruz NA; Programa de Investigación de Producción de Biomoléculas, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP. 70228, Mexico City, CP. 04510, Mexico.
Microb Cell Fact ; 18(1): 200, 2019 Nov 14.
Article em En | MEDLINE | ID: mdl-31727078
The global rise in urbanization and industrial activity has led to the production and incorporation of foreign contaminant molecules into ecosystems, distorting them and impacting human and animal health. Physical, chemical, and biological strategies have been adopted to eliminate these contaminants from water bodies under anthropogenic stress. Biotechnological processes involving microorganisms and enzymes have been used for this purpose; specifically, laccases, which are broad spectrum biocatalysts, have been used to degrade several compounds, such as those that can be found in the effluents from industries and hospitals. Laccases have shown high potential in the biotransformation of diverse pollutants using crude enzyme extracts or free enzymes. However, their application in bioremediation and water treatment at a large scale is limited by the complex composition and high salt concentration and pH values of contaminated media that affect protein stability, recovery and recycling. These issues are also associated with operational problems and the necessity of large-scale production of laccase. Hence, more knowledge on the molecular characteristics of water bodies is required to identify and develop new laccases that can be used under complex conditions and to develop novel strategies and processes to achieve their efficient application in treating contaminated water. Recently, stability, efficiency, separation and reuse issues have been overcome by the immobilization of enzymes and development of novel biocatalytic materials. This review provides recent information on laccases from different sources, their structures and biochemical properties, mechanisms of action, and application in the bioremediation and biotransformation of contaminant molecules in water. Moreover, we discuss a series of improvements that have been attempted for better organic solvent tolerance, thermo-tolerance, and operational stability of laccases, as per process requirements.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lacase / Poluentes Ambientais / Biocatálise Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lacase / Poluentes Ambientais / Biocatálise Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2019 Tipo de documento: Article