Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies.
Nat Methods
; 17(1): 50-54, 2020 01.
Article
em En
| MEDLINE
| ID: mdl-31740821
Spatially controlled cell adhesion on electron microscopy supports remains a bottleneck in specimen preparation for cellular cryo-electron tomography. Here, we describe contactless and mask-free photo-micropatterning of electron microscopy grids for site-specific deposition of extracellular matrix-related proteins. We attained refined cell positioning for micromachining by cryo-focused ion beam milling. Complex micropatterns generated predictable intracellular organization, allowing direct correlation between cell architecture and in-cell three-dimensional structural characterization of the underlying molecular machinery.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Manejo de Espécimes
/
Processamento de Imagem Assistida por Computador
/
Membrana Celular
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Microscopia Crioeletrônica
/
Proteínas de Fluorescência Verde
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Imagem Molecular
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article