Enhancing medial layer recellularization of tissue-engineered blood vessels using radial microchannels.
Regen Med
; 14(11): 1013-1028, 2019 11.
Article
em En
| MEDLINE
| ID: mdl-31746270
ABSTRACT
Aim:
Cell repopulation of tissue-engineered vascular grafts (TEVGs) from decellularized arterial scaffolds is limited by dense concentric tunica media layers which impede cells migrating radially between the layers. We aimed to develop and validate a new microneedle device to modify decellularized carotid arteries with radial microchannels to enhance medial layer repopulation. Material &methods:
Modified decellularized porcine arteries were seeded with rat mesenchymal stem cells using either standard longitudinal injection, or a dual vacuum-perfusion bioreactor. Mechanical tests were used to assess the arterial integrity following modification. Results &conclusion:
The method herein achieved radial recellularization of arteries in vitro without significant loss of mechanical integrity, Thus, we report a novel method for successful radial repopulation of decellularized carotid artery-based tissue-engineered vascular grafts.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Prótese Vascular
/
Engenharia Tecidual
/
Microtecnologia
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article