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Nanoharvesting of bioactive materials from living plant cultures using engineered silica nanoparticles.
Khan, M Arif; Wallace, William T; Sambi, Jatinder; Rogers, Dennis Trent; Littleton, John M; Rankin, Stephen E; Knutson, Barbara L.
Afiliação
  • Khan MA; Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY 40506, USA.
  • Wallace WT; Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY 40506, USA.
  • Sambi J; Naprogenix Inc., Lexington, KY, USA.
  • Rogers DT; Naprogenix Inc., Lexington, KY, USA.
  • Littleton JM; Naprogenix Inc., Lexington, KY, USA.
  • Rankin SE; Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY 40506, USA. Electronic address: stephen.rankin@uky.edu.
  • Knutson BL; Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY 40506, USA. Electronic address: bknut2@uky.edu.
Mater Sci Eng C Mater Biol Appl ; 106: 110190, 2020 Jan.
Article em En | MEDLINE | ID: mdl-31753369
Plant secondary metabolites are valuable therapeutics not readily synthesized by traditional chemistry techniques. Although their enrichment in plant cell cultures is possible following advances in biotechnology, conventional methods of recovery are destructive to the tissues. Nanoharvesting, in which nanoparticles are designed to bind and carry biomolecules out of living cells, offers continuous production of metabolites from plant cultures. Here, nanoharvesting of polyphenolic flavonoids, model plant-derived therapeutics, enriched in Solidago nemoralis hairy root cultures, is performed using engineered mesoporous silica nanoparticles (MSNPs, 165 nm diameter and 950 m2/g surface area) functionalized with both titanium dioxide (TiO2, 425 mg/g particles) for coordination binding sites, and amines (NH2, 145 mg/g particles) to promote cellular internalization. Intracellular uptake and localization of the nanoparticles (in Murashige and Skoog media) in hairy roots were confirmed by tagging the particles with rhodamine B isothiocyanate, incubating the particles with hairy roots, and quenching bulk fluorescence using trypan blue. Nanoharvesting of biologically active flavonoids was demonstrated by observing increased antiradical activity (using 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay) by nanoparticles after exposure to hairy roots (indicating general antioxidant activity), and by the displacement of the radio-ligand [3H]-methyllycaconitine from rat hippocampal nicotinic receptors by solutes recovered from nanoharvested particles (indicating pharmacological activity specific to S. nemoralis flavonoids). Post-nanoharvesting growth suggests that the roots are viable after nanoharvesting, and capable of continued flavonoid synthesis. These observations demonstrate the potential for using engineered nanostructured particles to facilitate continuous isolation of a broad range of biomolecules from living and functioning plant cultures.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dióxido de Silício / Nanopartículas Metálicas / Compostos Fitoquímicos Limite: Animals / Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dióxido de Silício / Nanopartículas Metálicas / Compostos Fitoquímicos Limite: Animals / Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article