Physical and chemical mutagens improved Sporotrichum thermophile, strain ST20 for enhanced Phytase activity.
Saudi J Biol Sci
; 26(7): 1485-1491, 2019 Nov.
Article
em En
| MEDLINE
| ID: mdl-31762614
ABSTRACT
OBJECTIVE:
Phosphorous is an essential micronutrient of plants and involved in critical biological functions. In nature, phosphorous is mostly present in immobilized inorganic mineral and in the fixed organic form including phytic acid and phosphoesteric compounds. However, the bioavailability of bound phosphorous could be enhanced by the use of phosphate solubilizing microorganisms such as bacteria and fungi. The phytases are widespread in an environment and have been isolated from different sources comprising bacteria and fungi.METHODOLOGY:
In current studies, we show the successful use of gamma rays and EMS (Ethyl Methane Sulphonate) mutagenesis for enhanced activity of phytases in a fungal strain Sporotrichum thermophile.RESULTS:
We report an improved strain ST2 that could produce a clear halo zone around the colony, up to 24â¯mm. The maximum enzymatic activity was found of 382â¯U/mL on pH 5.5. However, the phytase activity was improved to 387â¯U/ml at 45⯰C. We also report that the mutants produced through EMS showed the greater potential for phytase production.CONCLUSION:
The current study highlights the potential of EMS mutagenesis for strain improvement over physical mutagens.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article