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Cellular Factor XIII, a Transglutaminase in Human Corneal Keratocytes.
Orosz, Zsuzsanna Z; Bárdos, Helga; Shemirani, Amir H; Beke Debreceni, Ildikó; Lassila, Riitta; Riikonen, Antti S; Kremer Hovinga, Johanna A; Seiler, Theo G; van Dorland, Hendrika A; Schroeder, Verena; Boda, Zoltán; Nemes, László; Früh Eppstein, Beatrice; Nagy, Bence; Facskó, Andrea; Kappelmayer, János; Muszbek, László.
Afiliação
  • Orosz ZZ; Division of Clinical Laboratory Science, Department of Laboratory Medicine, University of Debrecen, Faculty of General Medicine, 4032 Debrecen, Hungary.
  • Bárdos H; Department of Ophthalmology, University of Szeged, 6720 Szeged, Hungary.
  • Shemirani AH; Department of Preventive Medicine, University of Debrecen, 4028 Debrecen, Hungary.
  • Beke Debreceni I; Division of Clinical Laboratory Science, Department of Laboratory Medicine, University of Debrecen, Faculty of General Medicine, 4032 Debrecen, Hungary.
  • Lassila R; Department of Laboratory Medicine, University of Debrecen, Faculty of General Medicine, 4032 Debrecen, Hungary.
  • Riikonen AS; Coagulation Disorders Unit, Department of Hematology, Comprehensive Cancer Center, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland.
  • Kremer Hovinga JA; Department of Ophthalmology, University of Helsinki and Helsinki University Hospital, 00014 Helsinki, Finland.
  • Seiler TG; Department of Hematology and Central Hematology Laboratory, Inselspital, Bern University Hospital, and Department for BioMedical Research, University of Bern, 3008 Bern, Switzerland.
  • van Dorland HA; Universitätsklinik für Augenheilkunde, Inselspital, Universität Bern, 3010 Bern, Switzerland.
  • Schroeder V; Department of Hematology and Central Hematology Laboratory, Inselspital, Bern University Hospital, and Department for BioMedical Research, University of Bern, 3008 Bern, Switzerland.
  • Boda Z; Department for BioMedical Research (DBMR), Experimental Haemostasis Group, University of Bern, 3008 Bern, Switzerland.
  • Nemes L; Department of Internal Medicine, Thrombosis and Haemostasis Center, University of Debrecen, 4032 Debrecen, Hungary.
  • Früh Eppstein B; National Haemophilia Center and Haemostasis Department, State Health Center, 1134 Budapest, Hungary.
  • Nagy B; Universitätsklinik für Augenheilkunde, Inselspital, Universität Bern, 3010 Bern, Switzerland.
  • Facskó A; Department of Pathology, University of Szeged, 6270 Szeged, Hungary.
  • Kappelmayer J; Department of Ophthalmology, University of Szeged, 6720 Szeged, Hungary.
  • Muszbek L; Department of Laboratory Medicine, University of Debrecen, Faculty of General Medicine, 4032 Debrecen, Hungary.
Int J Mol Sci ; 20(23)2019 Nov 27.
Article em En | MEDLINE | ID: mdl-31783511
ABSTRACT
Cellular factor XIII (cFXIII, FXIII-A2), a transglutaminase, has been demonstrated in a few cell types. Its main function is to cross-link proteins by isopeptide bonds. Here, we investigated the presence of cFXIII in cells of human cornea. Tissue sections of the cornea were immunostained for FXIII-A in combination with staining for CD34 antigen or isopeptide cross-links. Isolated corneal keratocytes were also evaluated by immunofluorescent microscopy and flow cytometry. FXIII-A in the corneal stroma was quantified by Western blotting. FXIII-A mRNA was detected by RT-qPCR. The cornea of FXIII-A-deficient patients was evaluated by cornea topography. FXIII-A was detected in 68 ± 13% of CD34+ keratocytes. Their distribution in the corneal stroma was unequal; they were most abundant in the subepithelial tertile. cFXIII was of cytoplasmic localization. In the stroma, 3.64 ng cFXIII/mg protein was measured. The synthesis of cFXIII by keratocytes was confirmed by RT-qPCR. Isopeptide cross-links were detected above, but not within the corneal stroma. Slight abnormality of the cornea was detected in six out of nine FXIII-A-deficient patients. The presence of cFXIII in human keratocytes was established for the first time. cFXIII might be involved in maintaining the stability of the cornea and in the corneal wound healing process.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fator XIII / Transglutaminases / Substância Própria / Ceratócitos da Córnea Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fator XIII / Transglutaminases / Substância Própria / Ceratócitos da Córnea Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article