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The Clinical Implication of Vitamin D Nanomedicine for Peritoneal Dialysis-Related Peritoneal Damage.
Lee, Yi-Che; Huang, Chih-Ting; Cheng, Fong-Yu; Hung, Shih-Yuan; Lin, Tsun-Mei; Tsai, Yen-Chang; Chen, Chih-I; Wang, Hao-Kuang; Lin, Chi-Wei; Liou, Hung-Hsiang; Chang, Min-Yu; Wang, Hsi-Hao; Chiou, Yuan-Yow.
Afiliação
  • Lee YC; Division of Nephrology, Department of Internal Medicine, E-DA Dachang Hospital/ I-Shou University, Kaohsiung, Taiwan.
  • Huang CT; School of Medicine, College of Medicine.
  • Cheng FY; Division of Nephrology, Department of Internal Medicine, E-DA Hospital/ I-Shou University, Kaohsiung, Taiwan.
  • Hung SY; Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • Lin TM; Department of Chemistry, Chinese Culture University, Taipei, Taiwan.
  • Tsai YC; Division of Nephrology, Department of Internal Medicine, E-DA Hospital/ I-Shou University, Kaohsiung, Taiwan.
  • Chen CI; School of Medicine for International Students.
  • Wang HK; Department of Laboratory Medicine.
  • Lin CW; Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • Liou HH; Division of Colorectal Surgery, Department of Surgery.
  • Chang MY; Department of Neurosurgery.
  • Wang HH; Department of Medical Education, E-DA Hospital/ I-Shou University, Kaohsiung, Taiwan.
  • Chiou YY; Division of Nephrology, Department of Medicine, Hsin-Jen Hospital, New Taipei City, Taiwan.
Int J Nanomedicine ; 14: 9665-9675, 2019.
Article em En | MEDLINE | ID: mdl-31824158
ABSTRACT

PURPOSE:

Vitamin D is a novel potential therapeutic agent for peritoneal dialysis (PD)-related peritoneal fibrosis, but it can induce hypercalcemia and vascular calcification, which limits its applicability. In this study, we create nanotechnology-based drug delivery systems to investigate its therapeutics and side effects. MATERIALS AND

METHODS:

1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N- [amino-(polyethylene glycol)2000] (DSPE-PEG) and L-α-phosphatidylcholine (PC), which packages with 1α,25(OH)2D3, were used to construct vitamin D nanoliposomes. To confirm the function and safety of vitamin D nanoliposomes, peritoneal mesothelial cells were treated with TGF-ß1 and the reverse was attempted using vitamin D nanoliposomes. Antibodies (Ab) against the peritoneum-glycoprotein M6A (GPM6A) Ab were conjugated with vitamin D nanoliposomes. These particles were implanted into mice by intraperitoneal injection and the animals were monitored for the distribution and side effects induced by vitamin D.

RESULTS:

Vitamin D nanoliposomes were taken up by the mesothelial cells over time without cell toxicity and it also provided the same therapeutic effect in vitro. In vivo study, fluorescent imaging showed vitamin D nanoliposomes allow specific peritoneum target effect and also ameliorate vitamin D side effect.

CONCLUSION:

Nanoliposomes vitamin D delivery systems for the prevention of PD-related peritoneal damage may be a potential clinical strategy in the future.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peritônio / Vitamina D / Diálise Peritoneal / Nanomedicina Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peritônio / Vitamina D / Diálise Peritoneal / Nanomedicina Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article