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Lipid Droplet Accumulation in Human Pancreatic Islets Is Dependent On Both Donor Age and Health.
Tong, Xin; Dai, Chunhua; Walker, John T; Nair, Gopika G; Kennedy, Arion; Carr, Rotonya M; Hebrok, Matthias; Powers, Alvin C; Stein, Roland.
Afiliação
  • Tong X; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN.
  • Dai C; Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt, University Medical Center, Nashville, TN.
  • Walker JT; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN.
  • Nair GG; Diabetes Center, Department of Medicine, University of California, San Francisco, San Francisco, CA.
  • Kennedy A; Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC.
  • Carr RM; Division of Gastroenterology, Department of Medicine, Perelman School of Medicine, University of Pennsylvania Philadelphia, PA.
  • Hebrok M; Diabetes Center, Department of Medicine, University of California, San Francisco, San Francisco, CA.
  • Powers AC; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN.
  • Stein R; Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt, University Medical Center, Nashville, TN.
Diabetes ; 69(3): 342-354, 2020 03.
Article em En | MEDLINE | ID: mdl-31836690
Human but not mouse islets transplanted into immunodeficient NSG mice effectively accumulate lipid droplets (LDs). Because chronic lipid exposure is associated with islet ß-cell dysfunction, we investigated LD accumulation in the intact human and mouse pancreas over a range of ages and states of diabetes. Very few LDs were found in normal human juvenile pancreatic acinar and islet cells, with numbers subsequently increasing throughout adulthood. While accumulation appeared evenly distributed in postjuvenile acinar and islet cells in donors without diabetes, LDs were enriched in islet α- and ß-cells from donors with type 2 diabetes (T2D). LDs were also found in the islet ß-like cells produced from human embryonic cell-derived ß-cell clusters. In contrast, LD accumulation was nearly undetectable in the adult rodent pancreas, even in hyperglycemic and hyperlipidemic models or 1.5-year-old mice. Taken together, there appear to be significant differences in pancreas islet cell lipid handling between species, and the human juvenile and adult cell populations. Moreover, our results suggest that LD enrichment could be impactful to T2D islet cell function.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transplante das Ilhotas Pancreáticas / Ilhotas Pancreáticas / Diabetes Mellitus Tipo 2 / Células Secretoras de Glucagon / Células Secretoras de Insulina / Gotículas Lipídicas Tipo de estudo: Prognostic_studies Limite: Adolescent / Adult / Aged / Animals / Child / Child, preschool / Female / Humans / Infant / Male Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transplante das Ilhotas Pancreáticas / Ilhotas Pancreáticas / Diabetes Mellitus Tipo 2 / Células Secretoras de Glucagon / Células Secretoras de Insulina / Gotículas Lipídicas Tipo de estudo: Prognostic_studies Limite: Adolescent / Adult / Aged / Animals / Child / Child, preschool / Female / Humans / Infant / Male Idioma: En Ano de publicação: 2020 Tipo de documento: Article