The E3 protein of bovine coronavirus is a receptor-destroying enzyme with acetylesterase activity.
J Virol
; 62(12): 4686-90, 1988 Dec.
Article
em En
| MEDLINE
| ID: mdl-3184275
ABSTRACT
In addition to members of the Orthomyxoviridae and Paramyxoviridae, several coronaviruses have been shown to possess receptor-destroying activities. Purified bovine coronavirus (BCV) preparations have an esterase activity which inactivates O-acetylsialic acid-containing receptors on erythrocytes. Diisopropyl fluorophosphate (DFP) completely inhibits this receptor-destroying activity of BCV, suggesting that the viral enzyme is a serine esterase. Treatment of purified BCV with [3H]DFP and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the proteins revealed that the E3 protein was specifically phosphorylated. This finding suggests that the esterase/receptor-destroying activity of BCV is associated with the E3 protein. Furthermore, treatment of BCV with DFP dramatically reduced its infectivity in a plaque assay. It is assumed that the esterase activity of BCV is required in an early step of virus replication, possibly during virus entry or uncoating.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acetilesterase
/
Receptores Virais
/
Proteínas Virais
/
Glicoproteínas
/
Coronaviridae
Limite:
Animals
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article