No Difference Between Age-Matched Male and Female C57BL/6J Mice in Photopic and Scotopic Electroretinogram a- and b-Wave Amplitudes or in Peak Diurnal Outer Segment Phagocytosis by the Retinal Pigment Epithelium.

ABSTRACT

Mice provide informative models of enormous utility for eye research. Sex as biological variable must be considered when conducting studies exploring mouse models. To determine if sex confounds neural retina or retinal pigment epithelium (RPE) activity in wild-type C57BL/6J mice, we compared male and female mice with respect to retinal light response and RPE phagocytosis. We tested 2-month-old mice at peak fertility and 12-month-old mice past fertility. Retinal function was assessed by quantifying a- and b-wave amplitudes of photopic and scotopic electroretinograms (ERGs). These experiments did not reveal differences between male and female mice at either age. As expected from earlier studies, 12-month-old mice showed reduced light responses compared to 2-month-old mice, but age-related decline was identical for male and female mice. RPE functionality was assessed by quantifying RPE phagosome content 1 h after light onset in mice 2 months of age, an age of maturity of the process of outer segment turnover that includes RPE phagocytosis. These experiments did not reveal differences in RPE phagocytosis between male and female mice. Altogether, male and female C57BL/6J mice do not differ in retinal light response and peak RPE phagocytic activity. Retinal activity is impaired with age to the same extent in male and female mice. Our results justify testing mixed-sex mouse cohorts in studies on outer segment renewal and RPE phagocytosis and illustrate the importance of careful consideration of cohort age.