High-level constitutive expression of leech hyaluronidase with combined strategies in recombinant Pichia pastoris.

Huang, Hao; Liang, Qixing; Wang, Yang; Chen, Jian; Kang, Zhen

Huang, Hao; Liang, Qixing; Wang, Yang; Chen, Jian; Kang, Zhen.

Afiliação

Huang H; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
Liang Q; The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Wang Y; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
Chen J; The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Kang Z; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.

Appl Microbiol Biotechnol ; 104(4): 1621-1632, 2020 Feb.

Article em En | MEDLINE | ID: mdl-31907577

Assuntos

Hialuronoglucosaminidase/biossíntese; Sanguessugas/enzimologia; Pichia/metabolismo; Animais; Técnicas de Cultura Celular por Lotes; Reatores Biológicos; Hialuronoglucosaminidase/genética; Microbiologia Industrial; Sanguessugas/genética; Pichia/genética; Regiões Promotoras Genéticas; Sinais Direcionadores de Proteínas/genética; Fatores de Transcrição/genética

Palavras-chave

Amino acid tag; Constitutive expression; Leech hyaluronidase; Pichia pastoris; Transcription factor

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pichia / Hialuronoglucosaminidase / Sanguessugas Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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